OriGene

MCF2 Monoclonal Antibody (OTI3E4), TrueMAB™

Name: MCF2 Monoclonal Antibody (OTI3E4), TrueMAB\u2122
Brand: OriGene
SKU: CF812542

查看其他4个MCF2抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)

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MCF2 Antibody (CF812542) in WB

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCF2 (RC230610, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-MCF2. (1. Positive lysates LY433609 (100 µg) and LC433609 (20 µg) can be purchased separately from OriGene.

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产品信息

CF812542

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

OTI3E4

抗原

Human recombinant protein fragment corresponding to amino acids 872-1001 of human MCF2 produced in E.coli.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.3, with 8% trehalose

内含物

no preservative

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

靶标信息

Guanine nucleotide exchange factor (GEF) that modulates the Rho family of GTPases. Promotes the conversion of some member of the Rho family GTPase from the GDP-bound to the GTP-bound form. Isoform 1 exhibits no activity toward RHOA, RAC1 or CDC42. Isoform 2 exhibits decreased GEF activity toward CDC42. Isoform 3 exhibits a weak but significant activity toward RAC1 and CDC42. Isoform 4 exhibits significant activity toward RHOA and CDC42. The truncated DBL oncogene is active toward RHOA, RAC1 and CDC42.

仅用于科研。不用于诊断过程。未经明确授权不得转售。