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Invitrogen
MCP-4 Monoclonal Antibody (3G4)
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MCP-4 Antibody (M809) in WB
Western blot analysis of human Monocyte Chemotactic Protein-4 (MCP-4)/CCL13 was performed by loading 2.5 µg of recombinant human MCP-4 protein per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with StartingBlock (TBS) Blocking Buffer (Product # 37542) for at least 1 hour. The membrane was probed with an MCP4 monoclonal antibody (Product # M809) at a concentration of 2 µg/mL overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween-20, and probed with a goat anti-mouse IgG-HRP ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
M809
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
3G4
抗原
Recombinant CCL13 protein
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1.0 mg/mL
规格
200 µg
纯化类型
Protein A
保存液
PBS
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
There is one major form (long chain) and two minor forms (short chain and medium chain) of MCP-4 produced by differential signal peptide cleavage. Thus, the resulting MW of secreted MCP-4 ranges between ~8-9kD. Western blot analysis of recombinant MCP-4/CCL13 protein detects an ~17kD band, likely corresponding to a dimer of MCP-4.
The M809 MCP-4 antibody (clone 3G4) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M810 (biotinylated conjugate of clone 8C12). The M809 MCP-4 antibody (biotinylated conjugate of clone 3G4) has also been used successfully in a sandwich ELISA as a detection antibody when paired with M810 (clone 8C12) as the coating antibody.
靶标信息
Monocyte Chemotactic Protein 4 (MCP-4), also called CCL13, is induced by inflammatory proteins such as IL-1 and TNFalpha. MCP-4 is a ligand for three different G protein coupled receptors, CCR2, CCR3 and CCR5. MCP-4 activates signaling in monocytes, T lymphocytes, eosinophils and basophils and this signaling is associated with the allergic response.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: C-C motif chemokine 13; CCL 13; CK-beta-10; H-MCP-4; MCP-4; Monocyte chemoattractant protein 4; Monocyte chemotactic protein 4; NCC-1; Small-inducible cytokine A13
基因别名: CCL13; MCP4; NCC1; SCYA13
Entrez Gene ID: (Human) 6357
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