Invitrogen

MICU1 Polyclonal Antibody

Name: MICU1 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-26686

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说明书

实验方案

技术支持

说明书

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MICU1 Antibody (PA5-26686) in WB

Western blot analysis using a CBARA1 polyclonal antibody (Product # PA5-26686) in K562 cell lysates (35 µg per lane).

Product Image Gallery

MICU1 Antibody — MDA-MB-231 mitochondrial Ca2+ influx is abrogated and enhanced by MCU and MICU1 knockdown, respectively. A. MDA-MB-231 mitochondria are capable of taking up Ca2+ in response extracellular stimulation. An example mt-Pericam recording from an adenovirally transduced cell during cellular stimulation with 100 µM ATP for the indicated duration. Mitochondrial Ca2+ levels were monitored from the ratio of its dual excitation absorbances (485 nm/415 nm) on emission (535 nm). B, C. Efficient down-regulation of MCU and MICU1 mRNA as determined by qPCR (B) and protein (C) after transient siRNA transfection. NC-si, negative control siRNA. For immunoblots (C), cells were fractionated into cytosolic and mitochondrial fractions prior to SDS-PAGE, as indicated. D. Fraction of mt-Pericam positive cells responding to 100 µM extracellular ATP application is significantly reduced after MCU knockdown (n?=?44, p<0.0001. Fisher's exact test) and increased after MICU1 knockdown (n?=?63, p<0.01) compared to NC-si (n?=?129). E. Average mt-Pericam signals in response to 100 µM ATP as indicated in siRNA transfected cells. Averages are shown as bold lines with SEM as fine lines. F, G. Summary data of mt-Pericam responses calculated for peak amplitude above baseline (F) and area under of the curve (G). Data are mean +/? SEM, t-test. {KD}

产品信息

PA5-26686

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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产品规格

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic peptide between 52-81 amino acids from the N-terminal region of human CBARA1

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2544186

产品详细信息

This antibody is predicted to react with non-human primate based on sequence homology.

靶标信息

MICU1 is a key regulator of mitochondrial calcium uptake required for calcium entry into mitochondrion. It may act as a calcium sensor via its EF-hand domains, gating the activity of a calcium channel partner. MICU1 induces T-helper 1-mediated autoreactivity, which is accompanied by the release of IFNG.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

MICU1 Polyclonal Antibody

MICU1 Antibody (PA5-26686) in WB

产品信息

PA5-26686

免疫印迹 (WB)

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

保存条件

运输条件

RRID

产品详细信息

靶标信息

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