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Invitrogen
MMP1 Polyclonal Antibody
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MMP1 Antibody (PA5-16498) in ICC/IF
Immunofluorescence analysis of MMP-1 (Collagenase-1) was performed using 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with MMP-1 (Collagenase-1) Rabbit Polyclonal Antibody (Product # 36-6300) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa F... View More
产品信息
PA5-16498
应用
建议稀释比
已发表文章
产品规格
已发表种属
C. elegans,
Chicken,
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide from the middle region of the human MMP-1
偶联物
形式
Liquid
浓度
1 mg/mL
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
PA5-16498 targets MMP-1 (Collagenase-1) in IHC (P) and WB applications and shows reactivity with Human samples. Antigen retrieval via boiling in 10 mM citrate buffer, pH 6.0 for 10 - 20 minutes followed by cooling to RT for 20 minutes is required for formalin fixed, paraffin embedded tissues.
The PA5-16498 immunogen is a synthetic peptide from the middle region of the human MMP-1.
靶标信息
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
