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Invitrogen
Maspin Polyclonal Antibody, Biotin, PeproTech®
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Maspin Antibody (500-P270BT-25UG) in WB
Western Blot: To detect hMaspin by Western Blot analysis Maspin Polyclonal Antibody, Biotin (Product # 500-P270BT-500UG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hMaspin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
500-P270BT-25UG
产品规格
种属反应
Human
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived Recombinant Human Maspin
偶联物
Biotin
Biotin
Biotin
形式
Lyophilized
浓度
0.1-1.0 mg/mL
规格
25 µg
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
RRID
产品详细信息
AA Sequence of recombinant protein: MDALQLANSA FAVDLFKQLC EKEPLGNVLF SPICLSTSLS LAQVGAKGDT ANEIGQVLHF ENVKDIPFGF QTVTSDVNKL SSFYSLKLIK RLYVDKSLNL STEFISSTKR PYAKELETVD FKDKLEETKG QINNSIKDLT DGHFENILAD NSVNDQTKIL VVNAAYFVGK WMKKFPESET KECPFRLNKT DTKPVQMMNM EATFCMGNID SINCKIIELP FQNKHLSMFI LLPKDVEDES TGLEKIEKQL NSESLSQWTN PSTMANAKVK LSIPKFKVEK MIDPKACLEN LGLKHIFSED TSDFSGMSET KGVALSNVIH KVCLEITEDG GDSIEVPGAR ILQHKDELNA DHPFIYIIRH NKTRNIIFFG KFCSP.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Maspin. Anti-Human Maspin-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hMaspin by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Maspin (500-P270) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hMaspin.
Western Blot: To detect hMaspin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hMaspin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P270BT-1MG will be provided as 2 x 500 µg
靶标信息
SERPINB5 is a tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: peptidase inhibitor 5; PI-5; protease inhibitor 5 (maspin); serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 5; serpin peptidase inhibitor, clade B (ovalbumin), member 5; SERPINB5-N; unnamed protein product
基因别名: maspin; PI5
UniProt ID: (Human) P36952
Entrez Gene ID: (Human) 5268
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