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Invitrogen
Myc Tag Monoclonal Antibody (Myc.A7), HRP
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Myc Tag Antibody (MA1-21316-HRP) in WB
Western blot analysis of Myc was performed by loading 50 µg of NCCIT and Jurkat lysates and 10 µg of Myc-MYCBP-containing cell lysate onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane. The membrane was blocked, and probed with an HRP-conjugated Myc Epitope Tag antibody (Product # MA1-21316-HRP) at a dilution of 1:1000 overnight at 4C. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075) at an exposure time of 2 minutes (NCCIT and Jurkat) and 3 seconds (Myc-MYCBP). Results show a ... View More
产品信息
MA1-21316-HRP
应用
建议稀释比
已发表文章
产品规格
种属反应
Tag
已发表种属
Tag
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
Myc.A7
抗原
Synthetic peptide: EQKLISEEDL (Myc)
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
0.07% Kathon
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
MA1-21316-HRP has been successfully used in Western blotting applications on myc tagged samples.
靶标信息
Myc is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
