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Invitrogen
NEIL2 Polyclonal Antibody
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NEIL2 Antibody (PA5-103829) in ICC/IF
Immunocytochemistry analysis of NEIL2 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with NEIL2 polyclonal antibody (Product # PA5-103829) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
产品信息
PA5-103829
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human NEIL2(Accession Q969S2), corresponding to amino acid residues G128-Y178.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Antibody detects endogenous levels of total NEIL2.
靶标信息
Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Has DNA glycosylase activity towards 5-hydroxyuracil and other oxidized derivatives of cytosine with a preference for mismatched double-stranded DNA (DNA bubbles). Has low or no DNA glycosylase activity towards thymine glycol, 2-hydroxyadenine, hypoxanthine and 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DNA glycosylase/AP lyase Neil2; DNA-(apurinic or apyrimidinic site) lyase Neil2; Endonuclease 8-like 2; Endonuclease VIII-like 2; NEH2; nei endonuclease VIII-like 2; Nei homolog 2; nei like 2; nei-like DNA glycosylase 2; Nei-like protein 2
基因别名: Gm1212; NEH2; NEI2; NEIL2
UniProt ID: (Human) Q969S2, (Mouse) Q6R2P8
Entrez Gene ID: (Human) 252969, (Mouse) 382913
damaged DNA binding
DNA-(apurinic or apyrimidinic site) lyase activity
protein binding
microtubule binding
zinc ion binding
DNA N-glycosylase activity
purine-specific mismatch base pair DNA N-glycosylase activity
nucleic acid binding
DNA binding
catalytic activity
uracil DNA N-glycosylase activity
hydrolase activity
hydrolase activity, acting on glycosyl bonds
hydrolase activity, hydrolyzing N-glycosyl compounds
lyase activity
single-strand selective uracil DNA N-glycosylase activity
DNA-3-methylbase glycosylase activity
metal ion binding
nicotinamide riboside hydrolase activity
nicotinic acid riboside hydrolase activity
deoxyribonucleoside 5'-monophosphate N-glycosidase activity
DNA metabolism protein
DNA glycosylase
nucleic acid metabolism protein
