Invitrogen

NF1 Polyclonal Antibody

查看其他11个NF1抗体

问题与答案

问题与答案

问题与答案

抗体检测数据 (2)

NF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 2

NF1 Antibody (PA1-46246) in IHC (P)

Immunohistochemistry analysis of NF1 in cartilage. Samples were incubated with NF1 polyclonal antibody (Product # PA1-46246). NF1 protein labeling displays positive signal in the maturing and the hypertrophic cartilages but only faint labeling in the proliferative cartilage.

NF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NF1 Antibody in Western Blot (WB)

Product Image Gallery

NF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of NF1 in cartilage. Samples were incubated with NF1 polyclonal antibody (Product # PA1-46246). NF1 protein labeling displays positive signal in the maturing and the hypertrophic cartilages but only faint labeling in the proliferative cartilage.

NF1 Antibody in Western Blot (WB) — Western blot analysis of NF1 in various lysates. Samples were incubated with NF1 polyclonal antibody (Product # PA1-46246). A: Nf1-/- knockout fibroblast cell extract; B: Normal rat Schwann cell extract; C: Normal human Schwann cell extract.

Immunogen image

NF1 Polyclonal Antibody

Immunogen Image NF1 Polyclonal Antibody

产品信息

PA1-46246

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

-

免疫组化（石蜡） (IHC (P))

1:200-1:500

-

免疫组化（冰冻） (IHC (F))

1:200-1:500

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

An N-terminal peptide of Neurofibromin 1 (within amino acids 1-50).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

Conc. Not Determined

保存液

whole serum

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

In Western blot, a band-set at 220-240 kDa and a higher MW band at 450 kDa (uncharacterized) can be seen.

For IHC, antigen retrieval is not required for immunostaining of frozen sections.

Antigen retrieval with trypsin or citrate improves immunostaining of paraffin sections.

靶标信息

The protein encoded by this gene is a type I integral membrane protein localized in the intermediate region between the endoplasmic reticulum and the Golgi, presumably recycling between the two compartments. The protein is a mannose-specific lectin and is a member of a novel family of plant lectin homologs in the secretory pathway of animal cells. Mutations in the gene are associated with a coagulation defect. Using positional cloning, the gene was identified as the disease gene leading to combined factor V-factor VIII deficiency, a rare, autosomal recessive disorder in which both coagulation factors V and VIII are diminished.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

篇参考文献 (0)

您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。

引用该产品

生物信息学

蛋白别名: DKFZp686J1293; FLJ21220; neurofibromatosis 1; Neurofibromatosis type 1; Neurofibromatosis-related protein NF-1; Neurofibromin

查看更多收起

基因别名: AW494271; E030030H24Rik; Nf-1; NF1; NFNS; VRNF; WSS

查看更多收起

UniProt ID: (Human) P21359, (Mouse) Q04690, (Rat) P97526

查看更多收起

Entrez Gene ID: (Human) 4763, (Mouse) 18015, (Rat) 24592

查看更多收起

GTPase activator activity protein binding phosphatidylethanolamine binding phosphatidylcholine binding microtubule binding lipid binding syndecan binding GTPase-activating protein

MAPK cascade osteoblast differentiation metanephros development response to hypoxia liver development negative regulation of endothelial cell proliferation positive regulation of endothelial cell proliferation regulation of cell-matrix adhesion negative regulation of cell-matrix adhesion negative regulation of protein kinase activity cell communication Ras protein signal transduction negative regulation of neuroblast proliferation brain development peripheral nervous system development heart development skeletal muscle tissue development visual learning extrinsic apoptotic signaling pathway via death domain receptors regulation of gene expression Schwann cell development phosphatidylinositol 3-kinase signaling negative regulation of angiogenesis spinal cord development amygdala development forebrain astrocyte development neural tube development cerebral cortex development myelination in peripheral nervous system actin cytoskeleton organization extracellular matrix organization collagen fibril organization adrenal gland development negative regulation of cell migration regulation of synaptic transmission, GABAergic cellular response to heat negative regulation of Rac protein signal transduction wound healing negative regulation of transcription factor import into nucleus positive regulation of apoptotic process regulation of GTPase activity negative regulation of MAP kinase activity negative regulation of MAPK cascade pigmentation positive regulation of neuron apoptotic process regulation of blood vessel endothelial cell migration positive regulation of GTPase activity regulation of bone resorption negative regulation of osteoclast differentiation regulation of glial cell differentiation positive regulation of adenylate cyclase activity regulation of angiogenesis negative regulation of Ras protein signal transduction negative regulation of neurotransmitter secretion negative regulation of fibroblast proliferation regulation of long-term neuronal synaptic plasticity sympathetic nervous system development camera-type eye morphogenesis negative regulation of astrocyte differentiation negative regulation of oligodendrocyte differentiation smooth muscle tissue development artery morphogenesis forebrain morphogenesis cognition gamma-aminobutyric acid secretion, neurotransmission glutamate secretion, neurotransmission observational learning regulation of long-term synaptic potentiation positive regulation of extrinsic apoptotic signaling pathway via death domain receptors positive regulation of extrinsic apoptotic signaling pathway in absence of ligand signal transduction negative regulation of cell proliferation regulation of cell proliferation regulation of MAPK cascade regulation of neuron differentiation