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Invitrogen
NFATC1 Monoclonal Antibody (7A6)
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NFATC1 Antibody (MA3-024) in ICC/IF
Immunofluorescent analysis of NFATc1 using NFATc1 Monoclonal Antibody (7A6) (Product # MA3-024) shows staining in MCF-7 Cells. NFATc1 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing NFATc1 (Product # MA3-024) at a dilution of 1:20 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35552 for GAR, P... View More
产品信息
MA3-024
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Non-human primate,
Rat
已发表种属
C. elegans,
Hamster,
Human,
Mouse,
Rabbit,
Rat
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
7A6
抗原
Bacterially expressed glutathione S-transferase (GST) fusion protein containing NF-ATc1 residues 1-654.
偶联物
形式
Liquid
浓度
Conc. Not Determined
保存液
ascites, PBS
内含物
0.05% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
MA3-024 detects NFATc1 (NFAT2) from transfected human, rat, mouse, and monkey cells. This antibody does not cross react with NFATc2 (NFAT1).
MA3-024 has been successfully used in Western blot, immunoprecipitation, ChIP, immunofluorescence and gel shift procedures. By Western blot, this antibody detects an ~105 kDa band representing transfected NFAT2 from COS cell lysate.
The MA3-024 immunogen is bacterially expressed glutathione S-transferase (GST) fusion protein containing NF-ATc1 residues 1-654.
Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell-based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization, including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody, may have to be performed for your high content assay.
靶标信息
The product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. The product of this gene is an inducible nuclear component. It functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. Five transcript variants encoding distinct isoforms have been identified for this gene. Different isoforms of this protein may regulate inducible expression of different cytokine genes.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: MGC138448; NF-ATc; NF-ATc1; NFAT tra; NFAT trans; NFAT transcription complex cytosolic component; nuclear factor of activated T-cells 'c'; Nuclear factor of activated T-cells, cytoplasmic 1; nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1; transcription factor NF-ATc
基因别名: 2210017P03Rik; AI449492; AV076380; NF-ATC; NF-ATc1.2; NFAT2; NFATC; NFATC1; Nfatcb
UniProt ID: (Human) O95644
Entrez Gene ID: (Human) 4772, (Rat) 100361818, (Mouse) 18018
RNA polymerase II distal enhancer sequence-specific DNA binding
RNA polymerase II transcription factor binding
transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding
transcription factor activity, sequence-specific DNA binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
protein binding
FK506 binding
mitogen-activated protein kinase p38 binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II core promoter sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II transcription coactivator binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
transcription factor binding
sequence-specific DNA binding
transcription regulatory region DNA binding
Rel homology transcription factor
transcription from RNA polymerase II promoter
Wnt signaling pathway, calcium modulating pathway
negative regulation of Wnt signaling pathway
calcineurin-NFAT signaling cascade
intracellular signal transduction
Fc-epsilon receptor signaling pathway
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
G1/S transition of mitotic cell cycle
negative regulation of transcription from RNA polymerase II promoter
epithelial to mesenchymal transition
lymphangiogenesis
B-1a B cell differentiation
heart valve morphogenesis
aortic valve morphogenesis
pulmonary valve morphogenesis
regulation of transcription from RNA polymerase II promoter
calcium ion transport
heart development
positive regulation of gene expression
transition between fast and slow fiber
osteoclast differentiation
regulation of hair cycle
negative regulation of osteoblast differentiation
ventricular septum morphogenesis
patterning of lymph vessels
heart trabecula morphogenesis
negative regulation of stem cell proliferation
transcription, DNA-templated
regulation of transcription, DNA-templated
cell quiescence
