Invitrogen

NKAP Polyclonal Antibody

查看其他6个NKAP抗体

问题与答案

问题与答案

问题与答案

抗体检测数据 (20)

NKAP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NKAP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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NKAP Antibody (PA5-115654) in IHC (P)

Immunohistochemistry analysis of NKAP in human pancreatic cancer and adjacent normal tissues. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-115654) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

NKAP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NKAP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NKAP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NKAP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NKAP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NKAP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NKAP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NKAP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NKAP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NKAP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NKAP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NKAP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NKAP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NKAP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NKAP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NKAP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NKAP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NKAP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NKAP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NKAP Antibody in Western Blot (WB)

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NKAP Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of NKAP in human pancreatic cancer and adjacent normal tissues. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-115654) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

NKAP Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of NKAP in human kidney cancer and adjacent normal tissues. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-115654) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

NKAP Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of NKAP in mouse muscle tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with NKAP polyclonal antibody (Product # PA5-115654) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

NKAP Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of NKAP in mouse colorectal tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-115654) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

NKAP Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of NKAP in rat pancreatic tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-115654) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

NKAP Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of NKAP in rat colorectal tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-115654) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

NKAP Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of NKAP in mouse stomach tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-115654) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

NKAP Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of NKAP in mouse testis tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-115654) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

NKAP Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of NKAP in human kidney cancer. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-115654) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

NKAP Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of NKAP in human kidney cancer. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-115654) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

NKAP Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of NKAP in rat ovarian tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-115654) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

NKAP Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of NKAP in mouse brain tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-115654) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

NKAP Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of NKAP in mouse liver tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-115654) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

NKAP Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of NKAP in mouse heart tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-115654) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

NKAP Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of NKAP in rat testis tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-115654) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

NKAP Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of NKAP in rat kidney tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-115654) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

NKAP Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of NKAP in mouse lung tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-115654) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

NKAP Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of NKAP in rat brain tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-115654) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

NKAP Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of NKAP in rat heart tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-115654) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

NKAP Antibody in Western Blot (WB) — Western blot analysis of NKAP in RAW264.7 cells (left lane: treated with blocking peptide). Samples were incubated with polyclonal antibody (Product # PA5-115654).

Immunogen image

NKAP Polyclonal Antibody

Immunogen Image NKAP Polyclonal Antibody

产品信息

PA5-115654

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:3,000

-

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human NKAP(Accession Q8N5F7), corresponding to amino acid residues G6-L56.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Antibody detects endogenous levels of total NKAP.

靶标信息

NKAP is a transcriptional repressor that associates with the NOTCH (see MIM 190198) corepressor complex and is required for T-cell development.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: FLJ22626; NF-kappa-B-activating protein; NF-kappaB activating protein; nuclear NF-kappaB activating protein

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基因别名: 2610020O08Rik; AA987160; AI849147; AL024236; NKAP

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UniProt ID: (Human) Q8N5F7, (Rat) Q4V7C9, (Mouse) Q9D0F4

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Entrez Gene ID: (Human) 79576, (Rat) 298342, (Mouse) 67050

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chromatin binding protein binding chromatin DNA binding poly(A) RNA binding

negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated Notch signaling pathway stem cell population maintenance granulocyte differentiation T cell differentiation in thymus negative regulation of transcription, DNA-templated positive regulation of alpha-beta T cell differentiation hematopoietic stem cell proliferation positive regulation of protein targeting to mitochondrion hemopoiesis regulation of transcription, DNA-templated