Invitrogen

NONO Polyclonal Antibody

Name: NONO Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-79748
Availability: InStock

高级验证

查看其他19个NONO抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (16)
高级验证 (2)

NONO Antibody in Immunocytochemistry (ICC/IF)

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NONO Antibody (PA5-79748) in ICC/IF

Immunocytochemistry analysis of nmt55/p54nrb using anti-nmt55/p54nrb antibody (Product # PA5-79748) . nmt55/p54nrb was detected in a section of SKOV-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-nmt55/p54nrb antibody (Product # PA5-79748) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section w... View More

NONO Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

NONO Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. PANC-1 cells were transfected with Non-POU domain-containing octamer-binding protein siRNA and decrease in signal intensity was observed in Western Blot application using Anti-NONO Polyclonal Antibody (Product # PA5-79748). {KD}

Immunogen image

Immunogen Image NONO Polyclonal Antibody

产品信息

PA5-79748

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

免疫细胞化学 (ICC/IF)

2 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide corresponding to a sequence at the N-terminus of human nmt55/p54nrb (1-35aa MQSNKTFNLEKQNHTPRKHHQHHHQQQHHQQQQQQ).

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

100 µg

纯化类型

Antigen affinity chromatography

保存液

PBS with 5mg BSA

内含物

0.05mg sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2746863

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human Hela whole cell, human A549 whole cell, human SW620 whole cell, human PANC-1 whole cell, human U20S whole cell, rat lung tissue, mouse lung tissue. IHC: mouse brain tissue, human lung cancer tissue, human intestinal cancer tissue, human lung cancer tissue, human mammary cancer tissue, rat intestine tissue. ICC/IF: U20S cell, SKOV-3 cell. Flow: HELA cell.

靶标信息

This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: 54 kDa nuclear RNA- and DNA-binding protein; 55 kDa nuclear protein; DNA-binding p52/p100 complex, 52 kDa subunit; HGNC:7871; NMT-1; NMT55; Non-POU domain-containing octamer-binding protein; non-POU-domain-containing; non-POU-domain-containing, octamer-binding protein; NonO protein; p54(nrb)

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基因别名: AA407051; AV149256; nonA; NONO; NRB54; P54NRB

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UniProt ID: (Mouse) Q99K48, (Rat) Q5FVM4

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Entrez Gene ID: (Mouse) 53610, (Rat) 317259

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功能

nucleotide binding core promoter binding nucleic acid binding DNA binding RNA binding protein binding identical protein binding poly(A) RNA binding RNA metabolism protein

参与通路