Invitrogen

NOP14 Polyclonal Antibody

查看其他7个NOP14抗体

问题与答案

问题与答案

问题与答案

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抗体检测数据 (2)

NOP14 Antibody in Immunocytochemistry (ICC/IF)

NOP14 Antibody in Immunocytochemistry (ICC/IF)

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NOP14 Antibody (PA5-103985) in ICC/IF

Immunocytochemistry analysis of NOP14 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with NOP14 polyclonal antibody (Product # PA5-103985) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

NOP14 Antibody in Immunocytochemistry (ICC/IF)

NOP14 Antibody in Western Blot (WB)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

NOP14 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of NOP14 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with NOP14 polyclonal antibody (Product # PA5-103985) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

NOP14 Antibody in Western Blot (WB) — Western blot analysis of NOP14 in Jurkat cell lysate. Samples were incubated with NOP14 polyclonal antibody (Product # PA5-103985).

Immunogen image

NOP14 Polyclonal Antibody

Immunogen Image NOP14 Polyclonal Antibody

产品信息

PA5-103985

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:3,000

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human NOP14(Accession P78316), corresponding to amino acid residues M338-S388.

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Antibody detects endogenous levels of total NOL14.

靶标信息

Involved in nucleolar processing of pre-18S ribosomal RNA. Has a role in the nuclear export of 40S pre-ribosomal subunit to the cytoplasm.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: NOP14 nucleolar protein homolog; Nucleolar complex protein 14; Nucleolar protein 14; probable nucleolar complex protein 14

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基因别名: 2610033H07Rik; C4orf9; NOL14; NOP14; RES4-25; RGD1305605

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UniProt ID: (Mouse) Q8R3N1

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Entrez Gene ID: (Mouse) 75416, (Rat) 289724

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enzyme binding poly(A) RNA binding RNA metabolism protein nucleic acid metabolism protein

rRNA processing biological_process maturation of SSU-rRNA ribosome biogenesis