Invitrogen

NOTCH2 (Cleaved Asp1733) Polyclonal Antibody

Name: NOTCH2 (Cleaved Asp1733) Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-99443
Availability: InStock

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (1)

NOTCH2 (Cleaved Asp1733) Antibody in Western Blot (WB)

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NOTCH2 (Cleaved Asp1733) Antibody (PA5-99443) in WB

Western blot analysis of NOTCH2 (Cleaved Asp1733) in 293 cell extract treated with TNF-alpha (left lane: treated with the antigen-specific peptide). Samples were incubated with NOTCH2 (Cleaved Asp1733) polyclonal antibody (Product # PA5-99443).

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

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产品信息

PA5-99443

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:100-1:500

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human NOTCH2(Accession Q04721), corresponding to amino acid residues L1717-S1741.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2818376

产品详细信息

Antibody detects endogenous levels of fragment of activated Notch2 resulting from cleavage adjacent to Asp1733.

靶标信息

NOTCH2 (Cleaved Asp1733) refers to the activated intracellular domain of the Notch2 receptor generated following gamma-secretase-mediated proteolytic cleavage at aspartic acid residue 1733. NOTCH2 is encoded by the NOTCH2 gene and functions as a single-pass transmembrane receptor in the conserved Notch signaling pathway, which regulates cell fate determination, differentiation, proliferation, and survival. Upon ligand engagement by Delta-like or Jagged family members on adjacent cells, NOTCH2 undergoes sequential proteolytic cleavages that culminate in intramembrane cleavage and release of the Notch2 intracellular domain (NICD2). The cleaved intracellular domain translocates to the nucleus, where it associates with the DNA-binding protein RBPJ and transcriptional co-activators to drive expression of target genes, including members of the HES and HEY families. Detection of NOTCH2 cleaved at Asp1733 is widely used as a marker of active Notch2 signaling in developmental biology, stem cell regulation, immunology, and cancer research.

仅用于科研。不用于诊断过程。未经明确授权不得转售。