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Invitrogen
NOXA1 Polyclonal Antibody
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NOXA1 Antibody (PA5-95084) in WB
Western blot analysis of NOXA1 using NOXA1 Polyclonal Antibody (Product # PA5-95084). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. Lane 1: human U87 whole cell lysates. Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membra... View More
产品信息
PA5-95084
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to a sequence in the middle region of human NOXA1 (176-195aa RQVPRGEVFRPHRWHLKHLE).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Affinity chromatography
保存液
PBS with 5mg BSA
内含物
0.05mg sodium azide, 0.05mg thimerosal
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human U87 whole cell, human Hela whole cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
NADPH oxidases (NOXs) catalyze the transfer of electrons from NADPH to molecular oxygen to generate reactive oxygen species (ROS). The NOX activator NOXA1 can stimulate both NOX1 and NOX2, but it appears to be more effective in activating NOX1.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Antigen NY-CO-31; inhibitory NADPH oxidase activator 1; NADPH oxidase activator 1; NCF2-like protein; NOX activator 1; p51-nox; P67phox-like factor; serologically defined colon cancer antigen 31
基因别名: NOXA1; NY-CO-31; P51NOX; SDCCAG31
UniProt ID: (Human) Q86UR1
Entrez Gene ID: (Human) 10811
