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Invitrogen
NPR1 Polyclonal Antibody
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FIGURE: 1 / 11
NPR1 Antibody (PA5-29049) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1) and tissue extracts of Rat Adipose (Lane 2). The blot was probed with Anti-NPR1 Polyclonal Antibody (Product # PA5-29049, 1:1000 dilution) and detected by chemiluminescence using Goat anti Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 118 kDa band corresponding to NPR1 was detected in the cell line and tissue tested. An uncharacterized band was also observed at ~20kDa in th... View More
Product Details
PA5-29049
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rabbit,
Rat
Published species
C. elegans,
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the Intracellular domain of human NPR-A. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: HL-60 membrane extracts.
Predicted reactivity: Mouse (95%), Rat (95%), Bovine (97%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Guanylyl cyclases, catalyzing the production of cGMP from GTP, are classified as soluble and membrane forms. The membrane guanylyl cyclases, often termed guanylyl cyclases A through F, form a family of cell-surface receptors with a similar topographic structure: an extracellular ligand-binding domain, a single membrane-spanning domain, and an intracellular region that contains a protein kinase-like domain and a cyclase catalytic domain. GC-A and GC-B function as receptors for natriuretic peptides; they are also referred to as atrial natriuretic peptide receptor A and type B. Also see NPR3, which encodes a protein with only the ligand-binding transmembrane and 37-amino acid cytoplasmic domains. NPR1 is a membrane-bound guanylate cyclase that serves as the receptor for both atrial and brain natriuretic peptides and BNP, respectively).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ANP-A; ANPR-A; atrial natriuretic peptide A-type receptor; Atrial natriuretic peptide receptor 1; atrial natriuretic peptide receptor A; Atrial natriuretic peptide receptor type A; atrionatriuretic peptide receptor A; GC-A; Guanylate cyclase A; natriuretic peptide A type receptor; natriuretic peptide receptor A; natriuretic peptide receptor A/guanylate cyclase A (atrionatriuretic peptide receptor A); NPR-A; testicular tissue protein Li 20
Gene Aliases: ANPa; ANPRA; Gca; GUC2A; GUCY2A; NPR-A; NPR1; NPRA
UniProt ID: (Human) P16066, (Rat) P18910
Entrez Gene ID: (Rabbit) 100343663, (Human) 4881, (Rat) 24603
cGMP biosynthetic process
protein phosphorylation
cell surface receptor signaling pathway
receptor guanylyl cyclase signaling pathway
G-protein coupled receptor signaling pathway
body fluid secretion
regulation of blood pressure
negative regulation of angiogenesis
negative regulation of cell growth
positive regulation of cGMP biosynthetic process
intracellular signal transduction
positive regulation of urine volume
positive regulation of renal sodium excretion
regulation of vasodilation
dopamine metabolic process
regulation of vascular permeability
negative regulation of smooth muscle cell proliferation
regulation of cardiac conduction
regulation of blood vessel size
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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