Invitrogen

NRF1 Monoclonal Antibody (2G4)

Name: NRF1 Monoclonal Antibody (2G4)
Brand: Invitrogen
SKU: MA5-36224

查看其他22个NRF1抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (21)

NRF1 Antibody in Immunocytochemistry (ICC/IF)

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NRF1 Antibody (MA5-36224) in ICC/IF

Immunocytochemistry analysis of NRF1 in MCF7 cells. Antigen retrieval was performed with enzyme antigen retrieval (15 min). Samples were blocked with 10% goat serum and incubated in NRF1 monoclonal antibody (Product # MA5-36224) at a dilution of 2 µg/mL (overnight, 4°C), followed by DyLight 488 conjugated goat anti-mouse IgG (30 min, 37°C) and DAPI at a dilution of 1:100.

NRF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

MA5-36224

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

免疫细胞化学 (ICC/IF)

2 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

2G4

抗原

E. coli-derived human NRF1 recombinant protein (Position: D246-Q503).

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Affinity chromatography

保存液

PBS with 4mg trehalose

内含物

0.05mg sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2884058

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human Jurkat whole cell, human HL-60 whole cell, human K562 whole cell, human Raji whole cell, human SH-SY5Y whole cell, human THP-1 whole cell. IHC: human rectal cancer tissue, human lung cancer tissue, human mammary cancer tissue, human testis cancer tissue, human testis cancer tissue, human testicular cancer tissue, human tonsil tissue, human gastric cancer tissue, human rectal cancer tissue, human appendicitis tissue, human appendicitis tissue, human bladder cancer tissue, human lung cancer tissue, human lung cancer tissue, human melanoma tissue, human ovarian cancer tissue, human renal carcinoma tissue, human skin cancer tissue. ICC/IF: MCF7 cell. Flow: PC-3 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

靶标信息

This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternate transcriptional splice variants, which encode the same protein, have been characterized. Additional variants encoding different protein isoforms have been described but they have not been fully characterized. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1.

仅用于科研。不用于诊断过程。未经明确授权不得转售。