MUbio

Neurofilament 160 kD Monoclonal Antibody (RNF403)

Name: Neurofilament 160 kD Monoclonal Antibody (RNF403)
Brand: MUbio
SKU: MUB1304P

查看其他19个NF-M抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

产品信息

MUB1304P

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:100-1:500

免疫组化（石蜡） (IHC (P))

1:50-1:100

免疫组化（冰冻） (IHC (F))

1:50-1:100

产品规格

种属反应

Hamster, Human, Non-human primate, Rat, Xenopus

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

RNF403

抗原

Neurofilament 160 kD

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A/G

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

RNF403 reacts exclusively with the phosphorylated isoform of the160 kD neurofilament protein.

The antibody may be stored at 4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

靶标信息

Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons. NFs, in most vertebrates, are composed of three different polypeptide chains with different molecular weights - neurofilament medium protein (NF-M), high (NF-H) and light protein (NF-L), which share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm. NF-M protein tail-mediated interactions of neurofilaments are critical for size and cytoskeletal architecture of axons, and are mediated, in part, by the highly phosphorylated tail domain of this protein. NF-M phosphorylation and O-GlcNAcylation are regulated reciprocally and affect its translocation and filament formation and function. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: 160 kDa neurofilament protein; middle molecular weight neurofilament protein NF-M(2); NEFM; Neurofilament 3; neurofilament 3, medium; Neurofilament medium polypeptide; Neurofilament protein, middle polypeptide; Neurofilament triplet M protein; neurofilament, medium polypeptide; neurofilament, medium polypeptide 150kDa; neurofilament, medium polypeptide S homeolog; neurofilament-3 (150 kD medium); NF-M; NMC

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基因别名: nef-m; NEF3; NEFM; nefm-a; nefm-b; NF-M; NFM; XELAEV_18020229mg

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UniProt ID: (Human) P07197, (Rat) P12839