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Invitrogen
Nitrotryptophan Monoclonal Antibody (2D12), FITC
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Nitrotryptophan Antibody (MA5-45818) in Flow
Flow Cytometry analysis using human neuroblastoma cells. Fixation involved 90% Methanol. Samples were incubated with Nitrotryptophan monoclonal antibody (Product # MA5-45818) at 1:50 for 30 min on ice, followed by Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 µM H2O2 for 24 hours.
产品信息
MA5-45818
应用
建议稀释比
已发表文章
产品规格
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
2D12
抗原
Synthetic 6-Nitrotryptophan modified Keyhole Limpet Hemocyanin (KLH).
偶联物
FITC
FITC
FITC
激发/发射光谱
498/517 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
规格
100 µg
保存条件
4°C, store in dark
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
A 1:1,000 dilution of MA5-45818 was sufficient for detection of 6-Nitrotryptophan in 1 µg of 6-Nitrotyrptophan conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.|Specific for 6-Nitrotyrptophan modified proteins. Doss not cross-react with 7-Ketocholoesterol modified proteins.
靶标信息
6-nitrotryptophan (6-NO2-Trp) is shown to be one of the most stable and major ONOO- modified amino acids, and growing evidences suggest that nitrotryptophan may play a significant role in the cellular regulation under nitrosative stress. Reactive nitrogen species (RNS) are formed in a variety of diseases such as atherosclerosis, inflammation, cancer and neurodegenerative disiases. Peroxinitrite (ONOO-) is known as a potent biological oxidant, which is formed via very fast reaction between nitric oxide (NO) and superoxide (O2-). Peroxinitrite can induce nitration of aromatic amino acids such as Tyr, Trp, Phe and His, which results in modulation of protein functions.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
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