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Invitrogen
ONECUT2 Polyclonal Antibody
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ONECUT2 Antibody (PA5-114396) in WB
Western blot analysis of ONECUT2 in the following samples: Lane 1: human K562 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human HepG2 whole cell lysates. Samples consisting of 50 µg (reducing conditions) of protein was separated with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V, Resolving gel; 2-3 hrs.), transferred to a Nitrocellulose membrane (150mA, 50-90 min) and washed with TBS-0.1% Tween (3 times, 5 minutes/wash) and blocked with 5% Non-fat Milk/TBS (1.5 hrs., room temperature). The membrane was incubated in ON... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-114396
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to a sequence of human ONECUT2 (MKAAYTAYRCLTKDLEGCAMNPELTMESLGTLH) (aa 1-33).
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
浓度
500 µg/mL
规格
100 µg
纯化类型
Affinity chromatography
保存液
PBS with 4mg trehalose
内含物
0.05mg sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human K562 whole cell, human HEK293 whole cell, human HepG2 whole cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
ONECUT2 is a member of the transcription factors of the ONECUT class, whose prototype is hepatocyte nuclear factor (HNF)-6. The distribution of OC-2 mRNA in humans is tissue-restricted, the strongest expression being detected in the liver and skin. ONECUT2 participates in the network of transcription factors required for liver differentiation and metabolism.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Hepatocyte nuclear factor 6-beta; HNF-6-beta; HNF-6B; OC-2; One cut domain family member 2; One cut homeobox 2; Transcription factor ONECUT-2; transcription factor ONECUT2
基因别名: C730009D12; HNF6B; OC-2; Oc2; ONECUT2
UniProt ID: (Mouse) Q6XBJ3
Entrez Gene ID: (Mouse) 225631
liver development
regulation of cell-matrix adhesion
epithelial cell development
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
anatomical structure morphogenesis
cell differentiation
positive regulation of cell migration
negative regulation of transforming growth factor beta receptor signaling pathway
pancreas development
endocrine pancreas development
cilium assembly
cell fate commitment
positive regulation of transcription from RNA polymerase II promoter
peripheral nervous system neuron development
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