Invitrogen

Occludin Monoclonal Antibody (OC-3F10), Alexa Fluor™ 488

Name: Occludin Monoclonal Antibody (OC-3F10), Alexa Fluor\u2122 488
Brand: Invitrogen
SKU: 331588

高级验证

14个已发表图片

26 篇参考文献

查看其他38个Occludin抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

Occludin Antibody in Immunocytochemistry (ICC/IF)

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Occludin Antibody (331588) in ICC/IF

Knockout of Occludin was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR777079_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Immunofluorescence analysis was performed on Caco-2 wild type cells (panel a,d), Caco-2 Cas9 control cells (panel b,e) and Caco-2 Occludin KO cells (panel c, f). Cells were fixed, permeabilized, and labeled with Occludin Monoclonal Antibody (OC-3F10), Alexa Fluor™ 488 (Product # 331588, 1:100). Nuclei (blue) were stained using ProLong™ Diamond Anti... View More

Occludin Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

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Immunogen image

Immunogen Image Occludin Monoclonal Antibody (OC-3F10), Alexa Fluor™ 488

产品信息

331588

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

查看2篇已发表文章 2篇已发表文章

免疫组化 (IHC)

查看5篇已发表文章 5篇已发表文章

免疫组织化学（多聚甲醛固定） (IHC (PFA))

查看1篇已发表文章 1篇已发表文章

免疫组化（冰冻） (IHC (F))

查看3篇已发表文章 3篇已发表文章

免疫组织化学（悬浮细胞） (IHC (Free))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1-5 µg/mL

查看14篇已发表文章 14篇已发表文章

酶联免疫吸附实验 (ELISA)

Assay-dependent

产品规格

种属反应

Dog, Human, Mouse, Rat

已发表种属

Bovine, C. elegans, Dog, Human, Mouse, Rat

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

OC-3F10

抗原

GST-fusion protein consisting of the C-terminal region (~150 aa) of human occludin.

查看免疫原

偶联物

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

激发/发射光谱

499/520 nm 查看光谱

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4, with 4.0mg/mL BSA

内含物

0.1% sodium azide

保存条件

4°C, store in dark

运输条件

Ambient (domestic); Wet ice (international)

AB_2532185

产品详细信息

Reactivity of this antibody with the occludin protein has been confirmed by immunofluorescence and western blotting (unconjugated). This antibody reacts specifically with mammalian occludin. Tissues/lysates that were tested with this antibody include T84 cell line (human intestinal epithelium), MDCK cells (canine kidney), Caco-2 cells (human colon adenocarcinoma), MTE7B (Mouse), and rat liver.

靶标信息

Occludin is a 65 kDa protein that can exist in a variety of phosphorylated forms, ranging up to approximately 82 kDa. Occludin is thought to be involved in regulating both the localization and the function of occludin. Polyunsaturated fatty acids are known to up-regulate occludin expression, increasing the transendothelial cell resistance and reducing the cellular permeability to large molecules. The level of occludin varies greatly depending on tissue; in brain tissue, occludin is highly and continuously expressed at cell-cell contact sites, whereas non-neural tissues show lower expression and discontinuous distribution. Overall structural features of the occludin protein are highly conserved in all the species examined. Under-expression of tight junction proteins, including occludin, is a key molecular abnormality responsible for the increased permeability of tumor endothelial tight junctions, which contributes to brain tumor edemas.

仅用于科研。不用于诊断过程。未经明确授权不得转售。