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Invitrogen
Osteopontin Polyclonal Antibody, Biotin, PeproTech®
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Osteopontin Antibody (500-P314BT-1MG) in WB
Western Blot: To detect Human Osteopontin by Western Blot analysis, Osteopontin Polyclonal Antibody, Biotin (Product # 500-P314BT-500UG) can be used at a concentration of 0.1-0.2 mg/mL. When used in conjunction with compatible development reagents, the detection limit for Recombinant Human Osteopontin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
产品信息
500-P314BT-1MG
产品规格
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived, 33.7 kDa Recombinant Human Osteopontin
偶联物
Biotin
Biotin
Biotin
形式
Lyophilized
浓度
0.1-1.0 mg/mL
保存条件
-20°C
运输条件
Ambient
RRID
产品详细信息
AA Sequence of recombinant protein: IPVKQADSGS SEEKQLYNKY PDAVATWLNP DPSQKQNLLA PQNAVSSEET NDFKQETLPS KSNESHDHMD DMDDEDDDDH VDSQDSIDSN DSDDVDDTDD SHQSDESHHS DESDELVTDF PTDLPATEVF TPVVPTVDTY DGRGDSVVYG LRSKSKKFRR PDIQYPDATD EDITSHMESE ELNGAYKAIP VAQDLNAPSD WDSRGKDSYE TSQLDDQSAE THSHKQSRLY KRKANDESNE HSDVIDSQEL SKVSREFHSH EFHSHEDMLV VDPKSKEEDK HLKFRISHEL DSASSEVN.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Osteopontin. Anti-Human Osteopontin-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human Osteopontin by sandwich ELISA (using 100 mL/well), a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti- Human Osteopontin (500-P314) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Osteopontin.
Western Blot: To detect Human Osteopontin by Western Blot analysis, this antibody can be used at a concentration of 0.1-0.2 mg/mL. When used in conjunction with compatible development reagents, the detection limit for Recombinant Human Osteopontin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P314BT-1MG will be provided as 2 x 500 µg
靶标信息
Osteopontin is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein that interacts with integrins and CD44 as major receptors. It is a multifunctional protein involved in bone mineralization, cell adhesion, cell migration, chronic inflammatory disease and transformation. Proteolytic cleavage by thrombin and matrix metalloproteinases close to the integrin-binding Arg-Gly-Asp sequence modulates the function of OPN and its integrin binding properties. Thrombin-cleaved fragments of Osteopontin are overexpressed in malignant glial tumors and provide a molecular niche with survival advantage and provide a novel substrate for plasmin and cathepsin D.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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