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Invitrogen
OxPhos Rodent WB Antibody Cocktail
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OxPhos Rodent WB Antibody (45-8099) in WB
Western blot analysis of OxPhos in Rat liver mitochondria using an OxPhos Monoclonal antibody cocktail (Product # 45-8099). The sample in lane 1 was kept at room temperature, whereas the remaining three samples were heated to 37°C, 50°C, and 100°C, respectively. This blot shows that boiling of samples leads to a decrease in signal due to aggregation of proteins therefore heating samples at or close to boiling is not recommended.
产品信息
45-8099
产品规格
种属反应
Bovine,
Human,
Mouse,
Non-human primate,
Rat
已发表种属
C. elegans,
Human,
Mouse,
Rat,
Sheep
宿主/亚型
Mouse
/ Ig
分类
Cocktail
类型
Antibody
克隆号
Cocktail
偶联物
形式
Liquid
浓度
1.5 mg/mL
纯化类型
purified
保存液
HEPES buffered saline
内含物
0.02% sodium azide
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
This product is a cocktail of multiple OxPhos antibodies.
C-I-20 (ND6) (clone) 20E9DH10C12 - Bovine Complex I
Purification Method: Antigen Affinity Chromatography
C-II-30 (FeS) (clone) 21A11AE7 - Bovine Heart Complex II
Purification Method: IgG Fraction
C-III-Core 2 (clone) 13G12AF12BB11 - Bovine Heart Complex II, III
Purification Method: IgG Fraction
C-IV-I (clone) 1D6E1A8 - Human Complex IV subunit I
Purification Method: Ammonium Sulfate Precipitation
C-V-alpha (clone) 15H4C4 - Bovine Complex V
Purification Method: Antigen Affinity Chromatography
The kit is suitable for Western Blotting analysis of the relative levels of the 5 OxPhos complexes in mitochondrial preparations from mouse, rat, human, or bovine sources. These clones were chosen because they are against a subunit that is labile when its complex is not assembled. Moreover, the combination is readily resolved in SDS-PAGE when the appropriate gel conditions are used. Also, COXI is a highly hydrophobic protein and appears as a broad band at ~35 kDa (not at its true molecular weight at 57 kDa). It is very sensitive to heating. Therefore, the samples, including the positive control, should not be heated over 50°C before loaded on the gel.
The antibody cocktail (1.5 mg/mL) should be diluted 250x to a final working concentration of 6.0 µg/mL for Western blotting. We recommend using PBS with 1% milk as the antibody diluent and using a high pH CAPS/PVDF transfer protocol.
靶标信息
Altered levels of assembly can arise from mutations in individual subunits, mutations in assembly factors for the complex(es), mtDNA depletion or as a result of physiological and or pathological changes e.g. hormone treatment, exercise, diet or oxidative stress.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
