Invitrogen

PABP Polyclonal Antibody

Name: PABP Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-17599
Availability: InStock

高级验证

1 篇参考文献

查看其他7个PABP抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (5)
高级验证 (1)

PABP Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 6

PABP Antibody (PA5-17599) in ICC/IF

Immunofluorescence analysis of PABP was performed using 70% confluent log phase HCT 116 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PABP Rabbit Polyclonal Antibody (Product # PA5-17599) at 1:250 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product... View More

PABP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PABP Antibody in RNA Immunoprecipitation (RIP)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

PABP Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with PABP siRNA and reduction of signal was observed in Western Blot using PABP Polyclonal Antibody. (Product # PA5-17599). {KD}

Immunogen image

Immunogen Image PABP Polyclonal Antibody

产品信息

PA5-17599

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100

免疫细胞化学 (ICC/IF)

1:250

RNA免疫共沉淀 (RIP)

2.5 µg/10^6 cells

产品规格

种属反应

Human, Mouse, Non-human primate, Rat

已发表种属

Zebrafish

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to amino acids at the amino-terminus of human PABP1

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

74 µg/mL

7.4 µg

纯化类型

Antigen affinity chromatography, Protein A

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

no preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_10980572

产品详细信息

It is not recommended to aliquot this antibody.

靶标信息

The poly(A)-binding protein (PABP), which is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation. In humans, the PABPs comprise a small nuclear isoform and a conserved gene family that displays at least 3 functional proteins: PABP1 (PABPC1), inducible PABP (iPABP, or PABPC4), and PABP3 (PABPC3). In addition, there are at least 4 pseudogenes, PABPCP1 to PABPCP4.

仅用于科研。不用于诊断过程。未经明确授权不得转售。