Invitrogen

PACSIN1 Polyclonal Antibody

Name: PACSIN1 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-90737

查看其他7个PACSIN1抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)

图: 1 / 1

PACSIN1 Antibody (PA5-90737) in WB

Western blot analysis of PACSIN1 in extracts of U-251MG cells. Samples were incubated with PACSIN1 Polyclonal antibody (Product # PA5-90737) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Product Image Gallery

Immunogen image

Immunogen Image PACSIN1 Polyclonal Antibody

产品信息

PA5-90737

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 240-390 of human PACSIN1 (NP_0658551)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2.08 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.01% thimerosal

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2806213

产品详细信息

Immunogen sequence: KRLVFLKEVL LDIKRHLNLA ENSSYIHVYR ELEQAIRGAD AQEDLRWFRS TSGPGMPMNW PQFEEWNPDL PHTTTKKEKQ PKKAEGVALT NATGAVESTS QAGDRGSVSS YDRGQPYATE WSDDESGNPF GGSETNGGAN PFEDDSKGVR V; Positive Samples: U-251MG; Cellular Location: Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoplasmic side, Cytoplasmic vesicle membrane, Membrane, Peripheral membrane protein, cytosol, ruffle membrane, synapse, synaptosome

靶标信息

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。