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Invitrogen
PAH Polyclonal Antibody
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PAH Antibody (PA5-79780) in IHC (P)
Immunohistochemistry (Paraffin) analysis of PAH in paraffin-embedded section of human liver cancer tissue using PAH Polyclonal Antibody (Product # PA5-79780). Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with the primary antibody at a 2 µg/mL dilution overnight at 4... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-79780
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E. coli-derived human PAH recombinant protein (Position: R71-H208).
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
浓度
500 µg/mL
规格
100 µg
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human HepG2 whole cell, human CACO-2 whole cell, rat kidney tissue, rat liver tissue, mouse kidney tissue, mouse liver tissue. IHC: human liver cancer tissue, mouse kidney tissue, rat kidney tissue. Flow: HepG2 cell.
靶标信息
PAH encodes the enzyme phenylalanine hydroxylase that is the rate-limiting step in phenylalanine catabolism. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: PAH; Phe-4-monooxygenase; Phenylalanine-4-hydroxylase
基因别名: AW106920; PAH
UniProt ID: (Mouse) P16331
Entrez Gene ID: (Mouse) 18478, (Rat) 24616
catalytic activity
monooxygenase activity
phenylalanine 4-monooxygenase activity
iron ion binding
oxidoreductase activity
amino acid binding
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced pteridine as one donor, and incorporation of one atom of oxygen
protein homodimerization activity
metal ion binding
cofactor binding
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