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Invitrogen
PASK Monoclonal Antibody (mAB6)
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图: 1 / 3
PASK Antibody (MA1-700) in ICC/IF
Immunofluorescent analysis of PASKIN (green) showing staining in the cytoplasm of COS7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a PASKIN monoclonal antibody (Product # MA1-700) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjug... View More
产品信息
MA1-700
应用
建议稀释比
已发表文章
产品规格
已发表种属
Human
宿主/亚型
Mouse
/ IgG2a
分类
Monoclonal
类型
Antibody
克隆号
mAB6
抗原
Recombinant, GST tagged, PAS fusion protein expressed in E. coli.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
100 µg
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
产品详细信息
MA1-700 contains 100 µg of Protein A/G purified, in vitro produced IgG2a (1 mg/mL) in PBS with 1 mg/mL BSA and 0.05% sodium azide.
MA1-700 detects PASK from human, non-human primate and rat samples.
MA1-700 has been successfully used in Western blot and CC/IF procedures.
The MA1-700 immunogen is recombinant, GST tagged, PAS fusion protein expressed in E. coli
This monoclonal antibody was expressed by hybridoma cells grown in vitro in ABR's dialysis-based bioreactor system. All media, reagents, and other variables that affect the system are continuously monitored and controlled, ensuring a superior degree of precision and repeatability compared to an in vivo ascites production. Additionally, the serum free medium is further protein purified for improved antibody performance.
靶标信息
PASK is an evolutionarily conserved protein present in yeast, flies, and mammals. PAS domains regulate the function of many intracellular signaling pathways in response to both extrinsic and intrinsic stimuli. It is a conserved gene product found in yeast as well as mammals. PASKIN contains two domains (PAS A and PAS B) as well as a serine/threonine kinase domain related to AMP kinases. PASKIN activity leads to decreased carbohydrate storage, as well as increased protein synthesis. PASKIN-dependent phosphorylation inhibits the activity of mammalian glycogen synthase.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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