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Invitrogen
PDE3B Recombinant Rat Monoclonal Antibody (SMCP3B)
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PDE3B Antibody (740069T) in WB
Western blot was performed using PDE3B Recombinant Rat Monoclonal Antibody (SMCP3B) (Product # 740069T) and ~124 kDa band corresponding to PDE3B was observed in 3T3-L1 adipocyte differentiated cells as compared to undifferentiated 3T3-L1 cell line, which is reported to have low PDE3B expression. Membrane-enriched extracts (40 µg lysate) of 3T3-L1 (Lane 1) and 3T3-L1 Adipocyte differentiated cells (Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel, 12-well (Product # NP0322BOX). Resolved proteins were then transferred on... View More
产品信息
740069T
产品规格
种属反应
Mouse
宿主/亚型
Rat
/ IgG2a, kappa
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
SMCP3B
偶联物
形式
Liquid
浓度
1.0 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
产品详细信息
Recombinant rat monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.
Applications Tested: This SMCP3B antibody has been tested in western blot and immunoprecipitation in 3T3-L1 Adipocyte differentiated cells. It can be used at 1 µg/mL in western blot and 10 µg in immunoprecipitation.
This antibody may result in detection of non-specific bands in western blot application along with the band of interest.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
靶标信息
Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. May play a role in fat metabolism. Regulates cAMP binding of RAPGEF3. Through simultaneous binding to RAPGEF3 and PIK3R6 assembles a signaling complex in which the PI3K gamma complex is activated by RAPGEF3 and which is involved in angiogenesis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CGI-PDE B; CGIPDE1; cGMP-inhibited 3',5'-cyclic phosphodiesterase 3B; cGMP-inhibited 3',5'-cyclic phosphodiesterase B; Cyclic GMP-inhibited phosphodiesterase B
基因别名: 9830102A01Rik; AI847709; Pde3b
UniProt ID: (Mouse) Q61409
Entrez Gene ID: (Mouse) 18576
3',5'-cyclic-nucleotide phosphodiesterase activity
3',5'-cyclic-AMP phosphodiesterase activity
cGMP-inhibited cyclic-nucleotide phosphodiesterase activity
phosphoric diester hydrolase activity
drug binding
hydrolase activity
cAMP binding
protein kinase B binding
metal ion binding
phosphoprotein binding
phosphodiesterase
