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PIF3/Phytochrome interacting factor 3 Polyclonal Antibody
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PIF3/Phytochrome interacting factor 3 Antibody (AS16-3954) in WB
Total protein from Arabidopsis thaliana (10-20 µg) of 4-d-old dark grown Col-0, pifsingle, triple and quadruple mutants were extracted with the buffer described in Qiu et al. 2015 and denatured at 95°C for 5 min. were separated on 8% Bis-Tris SDS-PAGE, and subsequently blotted to Nitrocellulose membrane using wet tank transfer. Blots were blocked with TBS containing 2% non-fat milk at room temperature for 1... View More
产品信息
AS16-3954
产品规格
种属反应
Plant
宿主/亚型
Goat
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide, derived from Arabidopsis thaliana PIF3.
偶联物
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
PIF3 is a nuclear-encoded transcription factor that accumulates in the nucleus and functions as a direct signaling partner of photoactivated phytochromes to couple light perception to transcriptional reprogramming during seedling development. The encoded protein is a basic helix-loop-helix regulator with a C-terminal bHLH DNA-binding/dimerization domain and an N-terminal PAS (Per-Arnt-Sim-like) region that supports protein-protein interactions, consistent with its role as a phytochrome-interacting factor. Functionally, PIF3 binds G-box-type cis elements in promoters of light-regulated genes and acts as a key modulator of photomorphogenesis and chloroplast biogenesis, contributing to rapid light-induced expression of nuclear genes needed for establishment of the photosynthetic apparatus at the dark-to-light transition while also repressing chlorophyll biosynthesis/photosynthesis programs in darkness through recruitment of chromatin repressors such as the histone deacetylase HDA15.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: F14J9.19; F14J9_19; PAP3; PHOTOCURRENT 1; PHYTOCHROME INTERACTING FACTOR 3; PHYTOCHROME-ASSOCIATED PROTEIN 3; POC1; purple acid phosphatase 3
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