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Agrisera
PIF5/Phytochrome interacting factor 5 Polyclonal Antibody
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PIF5/Phytochrome interacting factor 5 Antibody (AS12-2112) in WB
10 µg of total protein from 4 day old dark grown Arabidopsis thaliana seedlings extracted with hot buffer (Shen et al., Plant Cell. 2008; 20(6): 1,586-602) were separated on 8% SDS-PAGE and blotted 1 h to PVDF using semi-dry. Blots were blocked with the blocking buffer from Protein Detector Lumi GLO western blotting kit (KPL, Cat: 54-12-50) for 1 h at room temperature (RT) with agitation. Blot was incubated... View More
产品信息
AS12-2112
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana PIF5.
偶联物
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
PIF5 encodes a plant nuclear transcription factor that accumulates in darkness and functions as a central node in phytochrome-dependent light signaling in Arabidopsis. The protein is a basic helix-loop-helix DNA-binding factor with a C-terminal bHLH region that mediates sequence-specific binding to G-box class cis-elements in target promoters, and it carries an N-terminal active phytochrome B binding (APB) motif that confers selective interaction with the photoactivated phyB photoreceptor and supports rapid red-light-triggered phosphorylation and proteasome-dependent turnover. Functionally, PIF5 helps couple environmental light and photoperiod cues to transcriptional reprogramming that shapes early seedling development and growth, and it directly promotes dark-induced leaf senescence and chlorophyll catabolism by activating senescence-associated regulators and chlorophyll breakdown genes (for example via G-box-dependent control of ORE1 and SGR/NYC1 pathway components).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: A-PUT2; AT3G59060; AtbHLH65; BHLH065; bHLHb1; F17J16.110; PIL6
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