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Invitrogen
PIN1 Monoclonal Antibody (5E5)
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PIN1 Antibody (MA5-49154) in ICC/IF
Immunocytochemistry analysis of PIN1 in Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in PIN1 Monoclonal antibody (Product # MA5-49154) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for t... View More
产品信息
MA5-49154
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Non-human primate,
Rat
宿主/亚型
Mouse
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
5E5
抗原
E.coli-derived human Pin1 recombinant protein (Position: M1-E163).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Human Pin1 shares 95% amino acid (aa) sequence identity with mouse Pin1.
Positive Control - WB: human Hela whole cell, human HEK293 whole cell, human SW620 whole cell, monkey COS-7 whole cell, rat brain tissue, rat C6 whole cell. ICC/IF: Hela cell. Flow: U87 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
PIN1 (Peptidylprolyl Cis/Trans Isomerase, NIMA-Interacting 1) is a Protein Coding gene. Diseases associated with PIN1 include Meibomian Cyst and Viral Exanthem. Among its related pathways are Transport of the SLBP independent Mature mRNA and Cytokine Signaling in Immune system. Gene Ontology (GO) annotations related to this gene include peptidyl-prolyl cis-trans isomerase activity and mitogen-activated protein kinase binding.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: EC 5.2.1.8; NIMA-interacting; Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1; Peptidyl-prolyl cis-trans isomerase Pin1; PPIase Pin1; prolyl isomerase; protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1; Rotamase Pin1
基因别名: DOD; PIN1; UBL5
UniProt ID: (Human) Q13526
Entrez Gene ID: (Human) 5300, (Rat) 298696
protein peptidyl-prolyl isomerization
positive regulation of protein phosphorylation
cell cycle
regulation of mitotic nuclear division
neuron differentiation
negative regulation of transforming growth factor beta receptor signaling pathway
negative regulation of protein binding
regulation of cytokinesis
negative regulation of type I interferon production
positive regulation of protein dephosphorylation
negative regulation of protein catabolic process
negative regulation of neuron apoptotic process
positive regulation of neuron apoptotic process
positive regulation of GTPase activity
positive regulation of transcription from RNA polymerase II promoter
synapse organization
protein stabilization
positive regulation of ubiquitin-protein transferase activity
regulation of pathway-restricted SMAD protein phosphorylation
positive regulation of cell growth involved in cardiac muscle cell development
negative regulation of ERK1 and ERK2 cascade
positive regulation of canonical Wnt signaling pathway
regulation of protein localization to nucleus
regulation of signal transduction by p53 class mediator
negative regulation of cell motility
