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Invitrogen
PIWIL3 Polyclonal Antibody
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PIWIL3 Antibody (PA5-89073) in WB
Western blot analysis of PIWIL3 in extracts of various cell lines. Samples were incubated with PIWIL3 Polyclonal antibody (Product # PA5-89073) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
产品信息
PA5-89073
产品规格
种属反应
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 40-320 of human PIWIL3 (NP_0012429041)
偶联物
形式
Liquid
浓度
2.137 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.01% thimerosal
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Immunogen sequence: QLQSTPRPLQ EEVPVVRPLQ PRAARGGAGG GAQSQGVKEP GPEAGLHTAP LQERRIGGVF QDLVVNTRQD MKHVKDSKTG SEGTVVQLLA NHFRVISRPQ WVAYKYNVDY KPDIEDGNLR TILLDQHRRK FGERHIFDGN SLLLSRPLKE RRVEWLSTTK DKNIVKITVE FSKELTPTSP DCLRYYNILF RRTFKLLDFE QVGRNYYTKK KAIQLYRHGT SLEIWLGYVT SVLQYENSIT LCADVSHKLL RIETAYDFIK RTSAQAQTGN IREEVTNKLI G; Positive Samples: Mouse testis, Rat testis; Cellular Location: Cytoplasm
靶标信息
PIWI-L3 is a member of the PIWI subfamily of Argonaute proteins, evolutionarily conserved proteins containing both PAZ and Piwi motifs that are crucial for the biogenesis and function of small non-coding RNAs and play important roles in stem cell self-renewal, RNA silencing, and translational regulation in diverse organisms. Recent studies have shown that overexpression of PIWI-L3 (as well as other PIWI-like proteins) are potential biomarkers for astrocytic glioma, meningioma, and other cancers.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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