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Invitrogen
PLK2 Polyclonal Antibody
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PLK2 Antibody (PA5-79832) in ICC/IF
Immunocytochemistry analysis of PLK2 using anti-PLK2 antibody (Product # PA5-79832). PLK2 was detected in a section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 1μg/mL rabbit anti-PLK2 antibody (Product # PA5-79832) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as... View More
产品信息
PA5-79832
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to a sequence at the C-terminus of human PLK2 (666-685aa SSELKNRMEYALNMLLQRCN).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 5mg BSA
内含物
0.05mg sodium azide, 0.05mg thimerosal
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: A431 Cell, 293T Cell, COLO-320 Cell. IHC: human lung cancer tissue, rat intestine tissue, rat brain tissue. ICC/IF: A549 cell. Flow: A549 cell.
靶标信息
PLK2 is a serum inducible serine/threonine kinase that is a member of the polo family of serine/threonine kinases which have a role in normal cell division. The kinase may play a role in embryogenesis, wound healing, and neoplasia.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: h PLK-2; hSNK; PLK-2; polo-like kinase 1; Polo-like kinase 2; Serine/threonine-protein kinase PLK2; Serine/threonine-protein kinase SNK; Serum-inducible kinase
基因别名: hPlk2; hSNK; PLK2; SNK
UniProt ID: (Human) Q9NYY3, (Mouse) P53351, (Rat) Q9R012
Entrez Gene ID: (Human) 10769, (Mouse) 20620, (Rat) 83722
protein serine/threonine kinase activity
signal transducer activity
protein binding
ATP binding
protein complex binding
ATP-dependent protein binding
nucleotide binding
protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
protein modifying enzyme
G1/S transition of mitotic cell cycle
protein phosphorylation
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
mitotic spindle organization
mitotic cell cycle checkpoint
Ras protein signal transduction
memory
positive regulation of autophagy
peptidyl-serine phosphorylation
positive regulation of protein binding
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
Rap protein signal transduction
negative regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of protein catabolic process
regulation of centriole replication
regulation of synaptic plasticity
long-term synaptic potentiation
long term synaptic depression
negative regulation of dendritic spine development
mitotic cell cycle
phosphorylation
