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Invitrogen
PMCA ATPase Monoclonal Antibody (5F10)
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PMCA ATPase Antibody (MA3-914) in ICC/IF
Immunofluorescent analysis of PMCA ATPase using Anti-PMCA ATPase Monoclonal Antibody (5F10) (Product # MA3-914) shows staining in U251 Cells. PMCA ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PMCA ATP... View More
产品信息
MA3-914
应用
建议稀释比
已发表文章
产品规格
种属反应
Amphibian,
Dog,
Chicken,
Cat,
Human,
Mouse,
Non-human primate,
Sheep,
Rabbit,
Rat
已发表种属
Amphibian,
Bovine,
C. elegans,
Cat,
Dog,
Hamster,
Human,
Insect,
Mouse,
Non-human primate,
Pig,
Rabbit,
Rat
宿主/亚型
Mouse
/ IgG2a
分类
Monoclonal
类型
Antibody
克隆号
5F10
抗原
Purified human erythrocyte calcium ATPase.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
0.05% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
MA3-914 detects calcium pump of the plasma membrane (PMCA) ATPase from amphibian, canine, chicken, feline, human, mouse, sheep, primate, rabbit, and rat tissues. This antibody detects all four known isoforms of the PMCA ATPase.
MA3-914 has been successfully used in Western blot, FACS, immunohistochemistry, immunocytochemistry, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody recognizes an ~140 kDa protein representing PMCA ATPase and bands at 95kDa and 180 kDa which probably represent products of aggregation and/or natural proteolytic products of the pump from rat liver membrane preparations. Immunohistochemical staining of PMCA ATPase with MA3-914 yields a pattern consistent with that seen in the literature and depends on the tissue being studied and the localization of the isoforms present.
MA3-914 can be used on both frozen and formalin-fixed paraffin-embedded tissues.
The MA3-914 antigen is purified human erythrocyte calcium ATPase. This antibody recognizes an epitope between amino acids 724-783 of the human erythrocyte calcium pump.
靶标信息
The calcium pump of the plasma membrane (PMCA ATPase) pumps calcium from the cytosol to the extracellular space. This membrane-bound enzyme is related to a number of other ATPases including the sarcoplasmic and endoplasmic reticulum calcium (SERCA) ATPase and the sodium/potassium pump. Studies indicate that there are four known isoforms of the PMCA ATPase which are encoded by a multigene family.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: ATP-dependent plasma membrane calcium transporter; ATPase isoform 2 Na+K+ transporting beta polypeptide 2; ATPase isoform 2, Na+K+ transporting, beta polypeptide 2; ATPase, Ca++ transporting, plasma membrane 1; ATPase, Ca++ transporting, plasma membrane 2; ATPase, Ca++ transporting, plasma membrane 3; ATPase, Ca++ transporting, plasma membrane 4; Ca2+/Mg2+ ATPase; cilia and flagella associated protein 39; deaf waddler; Matrix-remodeling-associated protein 1; plasma membrane Ca(2+)-ATPase; plasma membrane Ca++-ATPase; plasma membrane Ca2+ pump 2; plasma membrane Ca2+-ATPases 4; plasma membrane calcium ATPase; Plasma membrane calcium ATPase isoform 1; Plasma membrane calcium ATPase isoform 2; Plasma membrane calcium ATPase isoform 3; Plasma membrane calcium ATPase isoform 4; plasma membrane calcium pump; Plasma membrane calcium pump isoform 1; Plasma membrane calcium pump isoform 2; Plasma membrane calcium pump isoform 3; Plasma membrane calcium pump isoform 4; Plasma membrane calcium-transporting ATPase 1; Plasma membrane calcium-transporting ATPase 2; Plasma membrane calcium-transporting ATPase 3; Plasma membrane calcium-transporting ATPase 4; PMCA1; PMCA1b; PMCA2; PMCA3; PMCA4; PMCA4a; PMCA4b; sarcolemmal calcium pump; wriggle
基因别名: 2810442I22Rik; 6430519O13Rik; ATP2B; ATP2B1; ATP2B2; ATP2B3; ATP2B4; CFAP39; CLA2; D6Abb2e; dfw; E130111D10Rik; Gena300; jog; MXRA1; OPCA; PMCA; PMCA1; Pmca1a; Pmca1b; Pmca1c; PMCA1kb; PMCA2; PMCA2a; PMCA2i; PMCA3; PMCA3a; PMCA4; PMCA4b; PMCA4x; SCAX1; Tmy; wms; wri
UniProt ID: (Human) P20020, (Rabbit) Q00804, (Chicken) Q98SH2, (Human) Q01814, (Human) Q16720, (Human) P23634, (Rat) P11505, (Mouse) G5E829, (Mouse) Q9R0K7, (Rat) P11506, (Rat) Q64568, (Mouse) Q6Q477, (Rat) Q64542
Entrez Gene ID: (Human) 490, (Rabbit) 100009543, (Sheep) 101111268, (Chicken) 374244, (Dog) 403480, (Sheep) 101121046, (Human) 491, (Chicken) 415958, (Dog) 476535, (Sheep) 101101873, (Human) 492, (Rabbit) 100356760, (Dog) 492238, (Human) 493, (Dog) 403731, (Rabbit) 100353865, (Chicken) 419934, (Rat) 29598, (Mouse) 67972, (Mouse) 11941, (Rat) 24215, (Mouse) 320707, (Rat) 29599, (Mouse) 381290, (Rat) 29600
calcium-transporting ATPase activity
protein binding
calmodulin binding
ATP binding
PDZ domain binding
metal ion binding
ATPase activity
calcium-dependent ATPase activity
sodium channel regulator activity
protein phosphatase 2B binding
nitric-oxide synthase inhibitor activity
nitric-oxide synthase binding
scaffold protein binding
nucleotide binding
hydrolase activity
calcium ion binding
protein C-terminus binding
acetylcholine receptor binding
glutamate receptor binding
primary active transporter
neural retina development
transport
cellular calcium ion homeostasis
brain development
aging
response to cold
ion transmembrane transport
calcium ion transmembrane transport
cellular response to vitamin D
cellular response to corticosterone stimulus
calcium ion export
regulation of cardiac conduction
regulation of cytosolic calcium ion concentration
calcium ion transport
organelle organization
lactation
sensory perception of sound
locomotory behavior
regulation of cell size
cerebellar Purkinje cell differentiation
cerebellar granule cell differentiation
neuron differentiation
locomotion
serotonin metabolic process
otolith mineralization
cGMP metabolic process
regulation of synaptic plasticity
synapse organization
neuromuscular process controlling balance
detection of mechanical stimulus involved in sensory perception of sound
positive regulation of calcium ion transport
auditory receptor cell stereocilium organization
cochlea development
regulation of transcription from RNA polymerase II promoter
spermatogenesis
negative regulation of nitric oxide mediated signal transduction
hippocampus development
sperm motility
positive regulation of peptidyl-serine phosphorylation
negative regulation of nitric oxide biosynthetic process
negative regulation of nitric-oxide synthase activity
response to hydrostatic pressure
negative regulation of calcineurin-NFAT signaling cascade
cellular response to epinephrine stimulus
calcium ion transmembrane import into cytosol
calcium ion import across plasma membrane
negative regulation of the force of heart contraction
negative regulation of arginine catabolic process
negative regulation of adrenergic receptor signaling pathway involved in heart process
negative regulation of peptidyl-cysteine S-nitrosylation
regulation of sodium ion transmembrane transport
regulation of cell cycle G1/S phase transition
negative regulation of cardiac muscle hypertrophy in response to stress
negative regulation of citrulline biosynthetic process
positive regulation of cAMP-dependent protein kinase activity
metabolic process
ion transport
cell morphogenesis
cerebellum development
cerebellar Purkinje cell layer morphogenesis
inner ear morphogenesis
inner ear development
inner ear receptor cell differentiation
