Invitrogen

POFUT1 Polyclonal Antibody

Name: POFUT1 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-31357

查看其他11个POFUT1抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (4)

POFUT1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 4

POFUT1 Antibody (PA5-31357) in ICC/IF

Immunocytochemistry analysis of POFUT1 in methanol-fixed HCT116. Samples were incubated with POFUT1 polyclonal antibody (Product # PA5-31357) using a dilution of 1:500.

Product Image Gallery

产品信息

PA5-31357

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:10,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein encompassing a sequence within the center region of human POFUT1. The exact sequence is proprietary.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.8 mg/mL

纯化类型

Antigen affinity chromatography

保存液

0.1M tris glycine, pH 7, with 20% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2548831

产品详细信息

Recommended positive controls: HCT116, mouse liver, HUVEC, HUVEC treat with Sphingosine 1-phosphate.

Predicted reactivity: Mouse (96%), Rat (97%), Dog (93%), Pig (95%), Chimpanzee (100%), Bovine (96%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

POFUT1, an endoplasmic reticulum-residing member of the glycosyltransferase O-Fuc family, adds O-fucose through an O-glycosidic linkage to conserved serine or threonines in epidermal growth factor-like repeats of several cell surface and secreted proteins. Unlike its homolog POFUT2, POFUT1 can also catalyze the transfer of fucose to thrombospondin type 1 repeats. Many of the substrates of POFUT1 are involved in ligand-induced receptor signaling. One such protein is Notch; mouse ES cells lacking POFUT have normal levels of Notch receptors at the cell surface, but these receptors do not bind Notch ligands or exhibit Notch signaling.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。