Invitrogen

PRKAR1A Monoclonal Antibody (OTI6C7)

Name: PRKAR1A Monoclonal Antibody (OTI6C7)
Brand: Invitrogen
SKU: MA5-24981

高级验证

查看其他16个PRKAR1A抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (13)
高级验证 (1)

PRKAR1A Antibody in Immunocytochemistry (ICC/IF)

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PRKAR1A Antibody (MA5-24981) in ICC/IF

Immunofluorescence analysis of PRKAR1A was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with PRKAR1A Mouse Monoclonal Antibody (OTI6C7) (Product # MA5-24981) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # ... View More

PRKAR1A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Immunogen Image PRKAR1A Monoclonal Antibody (OTI6C7)

产品信息

MA5-24981

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000

免疫组化（石蜡） (IHC (P))

1:50

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Human, Mouse

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

OTI6C7

抗原

Full-length protein expressed in 293T cell transfected with human PRKAR1A expression vector

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A/G

保存液

PBS, pH 7.3, with 1% BSA, 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2725102

靶标信息

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. This gene encodes one of the regulatory subunits. This protein was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Mutations in this gene cause Carney complex (CNC). This gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2. A nonconventional nuclear localization sequence (NLS) has been found for this protein which suggests a role in DNA replication via the protein serving as a nuclear transport protein for the second subunit of the Replication Factor C (RFC40). Three alternatively spliced transcript variants encoding the same protein have been observed.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: cAMP-dependent protein kinase regulatory subunit RIalpha; cAMP-dependent protein kinase type I-alpha regulatory chain; cAMP-dependent protein kinase type I-alpha regulatory subunit; Carney complex type 1; protein kinase A type 1a regulatory subunit; protein kinase, cAMP dependent regulatory, type 1, alpha; protein kinase, cAMP-dependent, regulatory subunit type I alpha; protein kinase, cAMP-dependent, regulatory, type I, alpha; Tissue-specific extinguisher 1; TSE1

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基因别名: 1300018C22Rik; ACRDYS1; ADOHR; CAR; CNC; CNC1; PKR1; PPNAD1; PRKAR1; PRKAR1A; RIalpha; Tse-1; TSE1

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UniProt ID: (Human) P10644, (Mouse) Q9DBC7

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Entrez Gene ID: (Human) 5573, (Mouse) 19084

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