Proteintech

PRMT5 Polyclonal Antibody

18 篇参考文献

查看其他25个PRMT5抗体

问题与答案

问题与答案

问题与答案

抗体检测数据 (15)

PRMT5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PRMT5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 15

PRMT5 Antibody (18436-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human lymphoma tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

PRMT5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PRMT5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PRMT5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PRMT5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PRMT5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PRMT5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PRMT5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PRMT5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PRMT5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PRMT5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PRMT5 Antibody in Western Blot (WB)

PRMT5 Antibody in Western Blot (WB)

PRMT5 Antibody in Western Blot (WB)

PRMT5 Antibody in Western Blot (WB)

PRMT5 Antibody in Western Blot (WB)

Product Image Gallery

PRMT5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lymphoma tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

PRMT5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lymphoma tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

PRMT5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 18436-1-AP ( PRMT5 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

PRMT5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 18436-1-AP ( PRMT5 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

PRMT5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:200 (under 40x lens).

PRMT5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:200 (under 10x lens).

PRMT5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:200 (under 40x lens).

PRMT5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:200 (under 10x lens).

PRMT5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using 18436-1-AP (PRMT5 Antibody) at dilution of 1:50 (under 40x lens).

PRMT5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using 18436-1-AP (PRMT5 Antibody) at dilution of 1:50 (under 10x lens).

PRMT5 Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with (Product # 18436-1-AP) PRMT5 antibody at dilution of 1:6,000 incubated at room temperature for 1.5 hours.

PRMT5 Antibody in Western Blot (WB) — Mouse embryo tissue were subjected to SDS PAGE followed by western blot with 18436-1-AP (PRMT5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

PRMT5 Antibody in Western Blot (WB) — HepG2 cells were subjected to SDS PAGE followed by western blot with 18436-1-AP ( PRMT5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

PRMT5 Antibody in Western Blot (WB) — Jurkat cells were subjected to SDS PAGE followed by western blot with 18436-1-AP (PRMT5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

PRMT5 Antibody in Western Blot (WB) — Raji cells were subjected to SDS PAGE followed by western blot with 18436-1-AP (PRMT5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Immunogen image

PRMT5 Polyclonal Antibody

Immunogen Image PRMT5 Polyclonal Antibody

产品信息

18436-1-AP

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

查看11篇已发表文章 11篇已发表文章

免疫组化 (IHC)

-

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

1:50-1:500

-

免疫沉淀 (IP)

Assay-dependent

查看5篇已发表文章 5篇已发表文章

产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

PRMT5 Fusion Protein Ag9736 (283-637 aa encoded by BC025979)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.18 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice (domestic); Dry ice (international)

产品详细信息

Immunogen sequence: YLEYLSQNR PPPNAYELFA KGYEDYLQSP LQPLMDNLES QTYEVFEKDP IKYSQYQQAI YKCLLDRVPE EEKDTNVQVL MVLGAGRGPL VNASLRAAKQ ADRRIKLYAV EKNPNAVVTL ENWQFEEWGS QVTVVSSDMR EWVAPEKADI IVSELLGSFA DNELSPECLD GAQHFLKDDG VSIPGEYTSF LAPISSSKLY NEVRACREKD RDPEAQFEMP YVVRLHNFHQ LSAPQPCFTF SHPNRDPMID NNRYCTLEFP VEVNTVLHGF AGYFETVLYQ DITLSIRPET HSPGMFSWFP ILFPIKQPIT VREGQTICVR FWRCSNSKKV WYEWAVTAPV CSAIHNPTGR SYTIGL (283-637 aa encoded by BC025979)

靶标信息

PRMT5 (protein arginine N-methyltransferase 5) is an arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. PRMT5 specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 and Sm D3; such methylation is required for the assembly and biogenesis of snRNP core particles.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: 72 kDa ICln-binding protein; EC 2.1.1.125; Histone-arginine N-methyltransferase PRMT5; HMT1 hnRNP methyltransferase-like 5; Jak-binding protein 1; PRMT5; Protein arginine N-methyltransferase 5; Shk1 kinase-binding protein 1 homolog; SKB1 homolog

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基因别名: HRMT1L5; IBP72; JBP1; PRMT5; SKB1; SKB1Hs

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UniProt ID: (Human) O14744, (Mouse) Q8CIG8

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Entrez Gene ID: (Human) 10419, (Mouse) 27374

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core promoter sequence-specific DNA binding transcription corepressor activity protein binding methyltransferase activity methyl-CpG binding histone-arginine N-methyltransferase activity protein-arginine N-methyltransferase activity protein complex binding protein-arginine omega-N symmetric methyltransferase activity ribonucleoprotein complex binding histone methyltransferase activity (H4-R3 specific) protein heterodimerization activity rRNA (adenine-N6,N6-)-dimethyltransferase activity chromatin binding protein C-terminal carboxyl O-methyltransferase activity tRNA (guanine-N2-)-methyltransferase activity C-methyltransferase activity N-methyltransferase activity O-methyltransferase activity S-methyltransferase activity RNA methyltransferase activity mRNA methyltransferase activity tRNA methyltransferase activity protein methyltransferase activity 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity rRNA methyltransferase activity rRNA (uridine-2'-O-)-methyltransferase activity rRNA (adenine-N6-)-methyltransferase activity DNA-methyltransferase activity tRNA (guanine-N1-)-methyltransferase activity rRNA (cytosine-C5-)-methyltransferase activity selenocysteine methyltransferase activity arginine N-methyltransferase activity lysine N-methyltransferase activity protein-lysine N-methyltransferase activity tRNA (uracil) methyltransferase activity tRNA (guanine) methyltransferase activity tRNA (adenine) methyltransferase activity tRNA (cytosine) methyltransferase activity tRNA (cytosine-5-)-methyltransferase activity tRNA (adenine-N1-)-methyltransferase activity rRNA (adenine) methyltransferase activity rRNA (cytosine) methyltransferase activity rRNA (guanine) methyltransferase activity rRNA (uridine) methyltransferase activity transferase activity protein C-terminal leucine carboxyl O-methyltransferase activity 1-phenanthrol methyltransferase activity protein-arginine N5-methyltransferase activity methylarsonite methyltransferase activity dimethylarsinite methyltransferase activity 4,5-dihydroxybenzo(a)pyrene methyltransferase activity 1-hydroxypyrene methyltransferase activity 1-hydroxy-6-methoxypyrene methyltransferase activity demethylmenaquinone methyltransferase activity cobalt-precorrin-6B C5-methyltransferase activity cobalt-precorrin-7 C15-methyltransferase activity cobalt-precorrin-5B C1-methyltransferase activity cobalt-precorrin-3 C17-methyltransferase activity dimethylamine methyltransferase activity hydroxyneurosporene-O-methyltransferase activity tRNA (adenine-57, 58-N(1)-) methyltransferase activity methylamine-specific methylcobalamin:coenzyme M methyltransferase activity trimethylamine methyltransferase activity methanol-specific methylcobalamin:coenzyme M methyltransferase activity monomethylamine methyltransferase activity P-methyltransferase activity Se-methyltransferase activity 2-phytyl-1,4-naphthoquinone methyltransferase activity tRNA (uracil-2'-O-)-methyltransferase activity tRNA (cytosine-2'-O-)-methyltransferase activity phosphomethylethanolamine N-methyltransferase activity tRNA (cytosine-3-)-methyltransferase activity rRNA (cytosine-2'-O-)-methyltransferase activity rRNA (cytosine-N4-)-methyltransferase activity trihydroxyferuloyl spermidine O-methyltransferase activity protein modifying enzyme

spliceosomal snRNP assembly DNA-templated transcription, termination regulation of transcription, DNA-templated regulation of mitotic nuclear division cell proliferation peptidyl-arginine methylation peptidyl-arginine methylation, to symmetrical-dimethyl arginine circadian regulation of gene expression peptidyl-arginine N-methylation endothelial cell activation histone H4-R3 methylation regulation of DNA methylation negative regulation of cell differentiation positive regulation of oligodendrocyte differentiation regulation of ERK1 and ERK2 cascade Golgi ribbon formation liver regeneration regulation of signal transduction by p53 class mediator negative regulation of nucleic acid-templated transcription positive regulation of adenylate cyclase-inhibiting dopamine receptor signaling pathway negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated protein methylation chromatin modification methylation histone arginine methylation rhythmic process