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Agrisera
PRP39a/pre-mRNA-processing factor 39 Polyclonal Antibody
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PRP39a/pre-mRNA-processing factor 39 Antibody (AS14-2784) in WB
40 µg of total protein from 14-day-old seedlings of Arabidopsis thaliana was extracted with extraction buffer containing: 100 m M Tris HCl, 10% glycerol, 5 m M EGTA, 0.15 M Na Cl, 0.75% Triton X 100,0.05% SDS, 1 m M DTT, 1 x Complete Mini EDTA-free protease inhibitor (Roche) were separated on 14% SDS/PAGE using semi-dry transfer and blotted 1 h to PVDF. Blots were blocked with 5% milk in TBS+0.1% Tween ON a... View More
产品信息
AS14-2784
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
Recombinant, full length PRP39a of Arabidopsis thaliana.
偶联物
形式
Lyophilized
保存液
whole serum
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
PRP39a encodes a conserved U1 snRNP-associated splicing factor that localizes predominantly to the nucleus, where it contributes to early spliceosome assembly during pre-mRNA processing. The protein is built largely from repeated tetratricopeptide repeat (TPR)-like helical motifs that form an extended scaffold for protein-protein interactions and can assemble as a homodimer, a configuration proposed to functionally substitute for the Prp39/Prp42 heterodimer found in fungi. Functionally, PRP39a promotes efficient 5-prime splice-site recognition by facilitating U1 snRNP engagement, with experimental evidence indicating a particular impact on usage of weak 5-prime splice sites and broad effects on alternative splicing patterns.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AT1G04080; F20D22.14; PRP39; PRP39-1
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