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Invitrogen
PTBP1 Polyclonal Antibody
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FIGURE: 1 / 4
PTBP1 Antibody (PA5-27429) in IHC (P)
hnRNP A1 Polyclonal Antibody detects PTBP1 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. PTBP1 stained by hnRNP A1 Polyclonal Antibody (Product # PA5-27429) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Product Details
PA5-27429
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human PTBP1. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: 293T, A431, HeLa, HepG2.
Predicted reactivity: Mouse (95%), Rat (95%), Japanese Medaka (82%), Xenopus laevis (91%), Pig (96%), Chicken (94%), Bovine (94%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has four repeats of quasi-RNA recognition motif (RRM) domains that bind RNAs. This protein binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway. It may also promote the binding of U2 snRNP to pre-mRNAs. This protein is localized in the nucleoplasm and it is also detected in the perinucleolar structure. Alternatively spliced transcript variants encoding different isoforms have been described.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 57 kDa RNA-binding protein PPTB-1; Heterogeneous nuclear ribonucleoprotein I; heterogeneous nuclear ribonucleoprotein polypeptide I; hnRNP I; MGC10830; MGC8461; polypyrimidine tract binding protein (heterogeneous nuclear ribonucleoprotein I); Polypyrimidine tract-binding protein 1; PTB; RNA-binding protein
Gene Aliases: AA407203; AL033359; HNRNP-I; HNRNPI; HNRPI; pPTB; PTB; PTB-1; PTB-T; PTB2; PTB3; PTB4; PTBP1
UniProt ID: (Human) P26599, (Mouse) P17225
Entrez Gene ID: (Human) 5725, (Mouse) 19205
regulation of alternative mRNA splicing, via spliceosome
mRNA splicing, via spliceosome
mRNA processing
RNA splicing
fibroblast growth factor receptor signaling pathway
gene expression
negative regulation of RNA splicing
negative regulation of mRNA splicing, via spliceosome
negative regulation of muscle cell differentiation
IRES-dependent viral translational initiation
positive regulation of transcription from RNA polymerase II promoter
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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