Invitrogen

PXMP4 Polyclonal Antibody

查看其他4个PXMP4抗体

问题与答案

问题与答案

问题与答案

抗体检测数据 (5)

PXMP4 Antibody in Immunocytochemistry (ICC/IF)

PXMP4 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 5

PXMP4 Antibody (PA5-118226) in ICC/IF

Immunofluorescence staining of PXMP4 in CACO2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with PXMP4 Polyclonal Antibody (Product # PA5-118226, 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

PXMP4 Antibody in Immunocytochemistry (ICC/IF)

PXMP4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PXMP4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PXMP4 Antibody in Western Blot (WB)

PXMP4 Antibody in Immunoprecipitation (IP)

Product Image Gallery

PXMP4 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence staining of PXMP4 in CACO2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with PXMP4 Polyclonal Antibody (Product # PA5-118226, 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

PXMP4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of human PXMP4 in human kidney with PXMP4 Polyclonal Antibody (Product # PA5-118226, 1:100 dilution, formalin-fixed paraffin embedded sections).

PXMP4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of human PXMP4 in human liver with PXMP4 Polyclonal Antibody (Product # PA5-118226, 1:100 dilution, formalin-fixed paraffin embedded sections).

PXMP4 Antibody in Western Blot (WB) — Western Blot using PXMP4 Polyclonal Antibody (Product # PA5-118226) at 1:500 dilution. Lane A: COLO205 Whole Cell Lysate, Lane B: NCI-H460 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 24 kDa. Observed band size: 24 kDa.

PXMP4 Antibody in Immunoprecipitation (IP) — PXMP4 Immunoprecipitation using: Lane A: 0.5 mg COLO205 Whole Cell Lysate 4 µL with PXMP4 Polyclonal Antibody (Product # PA5-118226) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: PXMP4 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 24 kDa. Observed band size: 24 kDa.

产品信息

PA5-118226

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

-

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

免疫沉淀 (IP)

1-5 µL/mg of lysate

-

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E. coli-derived Human PXMP4 fragment

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography, Protein A

保存液

PBS, pH 7

内含物

0.03% ProClin 300

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

PXMP4 belongs to the peroxisomal membrane protein PXMP2/4 family.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: 24 kDa peroxisomal intrinsic membrane protein; Peroxisomal membrane protein 4; peroxisomal membrane protein 4, 24kDa

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基因别名: PMP24; PXMP4

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UniProt ID: (Human) Q9Y6I8

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Entrez Gene ID: (Human) 11264

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protein binding

biological_process