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          • Primary Antibodies ›
          • AKT Pan Antibodies

          Invitrogen

          Phospho-AKT1/AKT2/AKT3 (Ser473) Recombinant Rabbit Monoclonal Antibody (PSH04-44)

          查看其他33个AKT Pan抗体

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          Cite Phospho-AKT1/AKT2/AKT3 (Ser473) Recombinant Rabbit Monoclonal Antibody (PSH04-44)

          Additional Information:
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          • 抗体检测数据 (5)
          Phospho-AKT1/AKT2/AKT3 (Ser473) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.
          Phospho-AKT1/AKT2/AKT3 (Ser473) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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          图: 1 / 5

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          Phospho-AKT1/AKT2/AKT3 (Ser473) Antibody (MA5-56569) in IHC (P)

          Immunohistochemistry analysis of Phospho-AKT1/AKT2/AKT3 (Ser473) with paraffin-embedded human breast cancer tissue. The tissues were blocked in 1% BSA (20 min, room temp), washed with ddH2O and PBS, incubated with Phospho-AKT1/AKT2/AKT3 (Ser473) Recombinant Monoclonal Antibody (Product # MA5-56569) at a dilution of 1:200 (1 hr, room temp). {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
          已发表图片由___提供 benchsci-logo
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          Phospho-AKT1/AKT2/AKT3 (Ser473) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-AKT1/AKT2/AKT3 (Ser473) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-AKT1/AKT2/AKT3 (Ser473) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-AKT1/AKT2/AKT3 (Ser473) Antibody in Western Blot (WB)
          Phospho-AKT1/AKT2/AKT3 (Ser473) Antibody in Flow Cytometry (Flow)
          Phospho-AKT1/AKT2/AKT3 (Ser473) Recombinant Rabbit Monoclonal Antibody (PSH04-44)

          产品信息

          MA5-56569

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          1:2,000-1:5,000
          -

          免疫组化(石蜡) (IHC (P))

          1:200
          -

          流式细胞分析 (Flow)

          1:10,000
          -
          产品规格

          种属反应

          Human, Mouse, Rat

          宿主/亚型

          Rabbit / IgG

          Expression System

          HEK293 cells

          分类

          Recombinant Monoclonal

          类型

          Antibody

          克隆号

          PSH04-44

          抗原

          Synthetic phosphopeptide corresponding to residues surrounding Ser473 of human Akt1.
          查看免疫原

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Liquid

          浓度

          1 mg/mL

          纯化类型

          Protein A

          保存液

          PBS, pH 7.4, with 0.1% BSA, 40% glycerol

          内含物

          0.05% sodium azide

          保存条件

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          运输条件

          Ambient (domestic); Wet ice (international)

          RRID

          AB_3679596

          靶标信息

          AKT also known as protein kinase B (PKB) or RAS-alpha, is an ubiquitous serine/threonine kinase that plays an important role in diverse biological responses such as regulation of metabolism, cell survival and growth by phosphorylating multiple proteins. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          篇参考文献 (0)

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          引用该产品

          生物信息学

          蛋白别名: AKT1 kinase; AKT1m; Akt1m protein; AKT2 kinase; AKT3 kinase; DKFZp434N0250; kinase Akt1; MGC99656; murine thymoma viral (v-akt) homolog-2; murine thymoma viral (v-akt) oncogene homolog 1; murine thymoma viral (v-akt) oncogene homolog 2; pan akt; PKB; PKB alpha; PKB beta; PKB gamma; PKBgamma/Akt3; PKBgamma1; Protein kinase Akt-2; Protein kinase Akt-3; Protein kinase B; Protein kinase B alpha; Protein kinase B beta; Protein kinase B gamma; protein kinase B, beta; protein kinase B, gamma; protein kinase B-alpha; Proto-oncogene c-Akt; putative v-akt murine thymoma viral oncoprotein 2; rac protein kinase alpha; RAC protein kinase alpha RAC-PK alpha; RAC protein kinase beta; RAC protein kinase beta RAC-PK beta; RAC-alpha serine/threonine-protein kinase; RAC-beta serine/threonine protein kinase; RAC-beta serine/threonine-protein kinase; RAC-gamma serine/threonine protein kinase; RAC-gamma serine/threonine-protein kinase; RAC-PK-alpha; RAC-PK-beta; RAC-PK-gamma; related to A and C kinases; serine-threonine protein kinase; similar to rat serine-threonine protein kinase; STK-2; Thymoma viral proto-oncogene; thymoma viral proto-oncogene 1; thymoma viral proto-oncogene 2; thymoma viral proto-oncogene 3; unnamed protein product; v-akt murine thymoma viral oncogene 3; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene homolog 2; v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma); v-akt murine thymoma viral oncogene-like protein 1

          查看更多 收起

          基因别名: 2410016A19Rik; AI851531; AKT; AKT1; AKT2; AKT3; AW554154; D930002M15Rik; HIHGHH; MPPH; MPPH2; Nmf350; PKB; PKB-ALPHA; PKB-GAMMA; PKB/Akt; PKBalpha; PKBB; PKBBETA; PKBG; PRKBA; PRKBB; PRKBG; RAC; RAC-ALPHA; RAC-BETA; RAC-gamma; RAC-PK-gamma; STK-2

          查看更多 收起

          UniProt ID: (Human) P31749, (Human) P31751, (Human) Q9Y243, (Mouse) P31750, (Rat) P47196, (Rat) P47197, (Mouse) Q60823, (Rat) Q63484, (Mouse) Q9WUA6

          查看更多 收起

          Entrez Gene ID: (Human) 207, (Human) 208, (Human) 10000, (Mouse) 11651, (Rat) 24185, (Rat) 25233, (Mouse) 11652, (Rat) 29414, (Mouse) 23797

          查看更多 收起

          功能
          nucleotide binding protein kinase activity protein serine/threonine kinase activity protein serine/threonine/tyrosine kinase activity protein binding calmodulin binding ATP binding phosphatidylinositol-3,4,5-trisphosphate binding kinase activity transferase activity enzyme binding kinase binding protein kinase binding nitric-oxide synthase regulator activity protein serine/threonine kinase inhibitor activity identical protein binding protein homodimerization activity phosphatidylinositol-3,4-bisphosphate binding 14-3-3 protein binding potassium channel activator activity protein serine kinase activity TORC2 complex binding metal ion binding molecular function activator activity protein kinase C binding transferase activity, transferring phosphorus-containing groups protein phosphatase 2A binding non-receptor serine/threonine protein kinase protein modifying enzyme
          参与通路
          osteoblast differentiation maternal placenta development positive regulation of endothelial cell proliferation cell migration involved in sprouting angiogenesis cytoplasmic translation complement receptor mediated signaling pathway sphingosine-1-phosphate signaling pathway glycogen metabolic process glycogen biosynthetic process regulation of glycogen biosynthetic process glucose metabolic process gluconeogenesis translational initiation regulation of translation protein phosphorylation ubiquitin-dependent protein catabolic process protein import into nucleus NLS-bearing protein import into nucleus nitric oxide biosynthetic process ER to Golgi vesicle-mediated transport Golgi to plasma membrane transport apoptotic process activation-induced cell death of T cells inflammatory response response to oxidative stress signal transduction epidermal growth factor receptor signaling pathway G-protein coupled receptor signaling pathway germ cell development nervous system development cell proliferation insulin receptor signaling pathway apoptotic mitochondrial changes cellular response to starvation response to heat response to hormone gene expression negative regulation of autophagy positive regulation of endothelial cell migration positive regulation of gene expression negative regulation of gene expression negative regulation of plasma membrane long-chain fatty acid transport fibroblast migration positive regulation of fibroblast migration positive regulation of sodium ion transport positive regulation of glucose metabolic process regulation of neuron projection development negative regulation of macroautophagy phosphorylation cell migration protein ubiquitination peptidyl-serine phosphorylation peptidyl-threonine phosphorylation cytokine-mediated signaling pathway oogenesis stage cell differentiation protein catabolic process positive regulation of cell growth regulation of cell migration positive regulation of cell migration negative regulation of cell migration anaphase-promoting complex-dependent catabolic process SCF-dependent proteasomal ubiquitin-dependent protein catabolic process T cell costimulation negative regulation of protein ubiquitination regulation of myelination lipopolysaccharide-mediated signaling pathway cellular response to nutrient levels TOR signaling negative regulation of fatty acid beta-oxidation positive regulation of endodeoxyribonuclease activity response to food peripheral nervous system myelin maintenance positive regulation of proteasomal ubiquitin-dependent protein catabolic process cellular response to insulin stimulus regulation of protein localization positive regulation of peptidyl-serine phosphorylation cellular response to stress negative regulation of toll-like receptor signaling pathway positive regulation of toll-like receptor signaling pathway toll-like receptor 4 signaling pathway cellular response to amino acid starvation response to fluid shear stress protein K29-linked ubiquitination intracellular signal transduction interleukin-18-mediated signaling pathway cellular response to vascular endothelial growth factor stimulus cellular response to decreased oxygen levels SREBP-SCAP complex retention in endoplasmic reticulum tRNA stabilization calcium ion transmembrane import into mitochondrion PERK-mediated unfolded protein response TORC1 signaling glucose homeostasis regulation of apoptotic process Golgi to plasma membrane protein transport negative regulation of apoptotic process proteasome-mediated ubiquitin-dependent protein catabolic process anoikis regulation of mRNA stability protein kinase B signaling positive regulation of blood vessel endothelial cell migration positive regulation of nitric oxide biosynthetic process positive regulation of cholesterol biosynthetic process positive regulation of fat cell differentiation positive regulation of cilium assembly positive regulation of glycogen biosynthetic process negative regulation of Notch signaling pathway negative regulation of proteolysis positive regulation of transcription from RNA polymerase II promoter negative regulation of translational initiation positive regulation of translational initiation nitric oxide metabolic process positive regulation of glucose import positive regulation of organ growth protein autophosphorylation positive regulation of lipid biosynthetic process insulin-like growth factor receptor signaling pathway behavioral response to pain positive regulation of smooth muscle cell proliferation striated muscle cell differentiation positive regulation of protein metabolic process protein maturation excitatory postsynaptic potential response to growth hormone regulation of vesicle-mediated transport mammary gland epithelial cell differentiation labyrinthine layer blood vessel development protein localization to cilium response to UV-A response to growth factor protein localization to endoplasmic reticulum protein K11-linked ubiquitination cellular response to calcium ion cellular response to tumor necrosis factor cellular response to growth factor stimulus cellular response to epidermal growth factor stimulus cellular response to prostaglandin E stimulus tricarboxylic acid metabolic process establishment of protein localization to mitochondrion maintenance of protein location in mitochondrion cellular response to rapamycin protein K6-linked ubiquitination negative regulation of release of cytochrome c from mitochondria cellular response to granulocyte macrophage colony-stimulating factor stimulus execution phase of apoptosis vascular endothelial cell response to laminar fluid shear stress regulation of postsynapse organization tRNA (guanine-N7)-methylation regulation of tRNA methylation cellular response to oxidised low-density lipoprotein particle stimulus cGAS/STING signaling pathway negative regulation of protein localization to lysosome negative regulation of cGAS/STING signaling pathway beta-arrestin-dependent dopamine receptor signaling pathway positive regulation of G1/S transition of mitotic cell cycle positive regulation of protein localization to nucleus cellular response to peptide regulation of signal transduction by p53 class mediator negative regulation of cilium assembly negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway negative regulation of leukocyte cell-cell adhesion positive regulation of protein localization to plasma membrane negative regulation of protein maturation negative regulation of hydrogen peroxide-induced neuron intrinsic apoptotic signaling pathway negative regulation of PERK-mediated unfolded protein response negative regulation of TORC1 signaling positive regulation of TORC1 signaling positive regulation of TORC2 signaling positive regulation of protein localization to endoplasmic reticulum positive regulation of anaphase-promoting complex-dependent catabolic process cellular response to nerve growth factor stimulus response to insulin-like growth factor stimulus positive regulation of protein localization to cell surface regulation of type B pancreatic cell development negative regulation of lymphocyte migration negative regulation of extrinsic apoptotic signaling pathway in absence of ligand negative regulation of intrinsic apoptotic signaling pathway positive regulation of fatty acid beta-oxidation protein modification process fat cell differentiation protein stabilization regulation of cell cycle cellular response to high light intensity protein localization to plasma membrane positive regulation of protein targeting to membrane retinal rod cell apoptotic process positive regulation of cap-dependent translational initiation positive regulation of cell motility mitochondrial genome maintenance positive regulation of TOR signaling positive regulation of angiogenesis positive regulation of cell size brain morphogenesis homeostasis of number of cells within a tissue positive regulation of cell migration involved in sprouting angiogenesis positive regulation of vascular endothelial cell proliferation positive regulation of artery morphogenesis negative regulation of cellular senescence protein import into nucleus, translocation positive regulation of protein phosphorylation carbohydrate metabolic process translation negative regulation of protein kinase activity transport cytoskeleton organization multicellular organism development aging carbohydrate transport negative regulation of endopeptidase activity glucose transport cell projection organization positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle positive regulation of cellular protein metabolic process anagen positive regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process negative regulation of cell size positive regulation of vasoconstriction negative regulation of JNK cascade positive regulation of nitric-oxide synthase activity positive regulation of sequence-specific DNA binding transcription factor activity glycogen cell differentiation involved in embryonic placenta development cellular response to mechanical stimulus cellular response to organic cyclic compound cellular response to hypoxia positive regulation of establishment of protein localization to plasma membrane hyaluronan metabolic process response to osmotic stress positive regulation of signal transduction response to muscle activity positive regulation of vesicle fusion response to nutrient levels response to insulin cellular response to hormone stimulus negative regulation of RNA splicing positive regulation of positive chemotaxis intracellular protein transmembrane transport positive regulation of glucose import in response to insulin stimulus activation of GTPase activity
          It has to be done as per old AB suggested Products section.

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