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Phospho-ATF2 (Tyr69, Thr71) Antibody (711221) in ICC/IF
For immunofluorescence analysis, PANC-1 cells were fixed and permeabilized for detection of endogenous ATF2 pT69/71 using ATF2 (pT69/71) Recombinant Rabbit Superclonal™ Antibody (Product # 711221, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using SlowFade® Gold Antifade Mountant... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
711221
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Superclonal
类型
Antibody
克隆号
24HCLC
抗原
Peptide corresponding to Human ATF2 (aa 66-75)
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
0.5 mg/mL
规格
100 µg
纯化类型
Protein A
保存液
PBS, pH 7.2-7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
产品详细信息
This antibody is predicted to react with Monkey, Dog, Sheep and Bovine
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
靶标信息
The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins. ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways. Various forms of cellular stress, including genotoxic agents, inflammatory cytokines and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71. Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Activating 2; ATF2; bZIP transcription factor; cAMP response element-binding protein CRE-BP1; cAMP responsive element binding protein 2, formerly; cAMP-dependent transcription factor ATF-2; cyclic AMP response element-binding protein (HB16); cyclic AMP-responsive element-binding protein 2; histone acetyltransferase ATF2; MGC111558; mXBP protein; OTTHUMP00000205345; OTTHUMP00000205347; OTTHUMP00000205348; OTTHUMP00000205350; OTTHUMP00000205352; OTTHUMP00000205353; OTTHUMP00000205354; unnamed protein product
基因别名: Atf-2; CRE-BP; CRE-BP1; CREB-2; CREB2; D130078H02Rik; HB16; mXBP; Tg(Gzma-Klra1)7Wum; TREB7
UniProt ID: (Human) P15336, (Mouse) P16951
Entrez Gene ID: (Human) 1386, (Mouse) 11909
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
histone acetyltransferase activity
protein binding
cAMP response element binding protein binding
H4 histone acetyltransferase activity
protein kinase binding
cAMP response element binding
identical protein binding
protein homodimerization activity
leucine zipper domain binding
sequence-specific DNA binding
histone H2B acetyltransferase activity
protein heterodimerization activity
RNA polymerase II sequence-specific DNA binding transcription factor binding
sequence-specific double-stranded DNA binding
promoter-specific chromatin binding
macromolecular complex binding
negative regulation of transcription from RNA polymerase II promoter
MAPK cascade
cellular glucose homeostasis
in utero embryonic development
NK T cell differentiation
liver development
positive regulation of protein phosphorylation
hematopoietic progenitor cell differentiation
outflow tract morphogenesis
brainstem development
growth plate cartilage chondrocyte differentiation
growth plate cartilage chondrocyte proliferation
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
protein import into nucleus
lipid metabolic process
apoptotic process
response to osmotic stress
cellular response to DNA damage stimulus
vacuole organization
JNK cascade
heart development
gene expression
positive regulation of gene expression
negative regulation of angiogenesis
abducens nucleus development
hypoglossal nucleus development
facial nucleus development
BMP signaling pathway
intra-S DNA damage checkpoint
positive regulation of transforming growth factor beta2 production
cellular response to oxidative stress
p38MAPK cascade
mRNA transcription from RNA polymerase II promoter
positive regulation of neuron apoptotic process
fat cell differentiation
positive regulation of gene expression, epigenetic
positive regulation of transcription from RNA polymerase II promoter
negative regulation of epithelial cell proliferation
white fat cell differentiation
neurofilament cytoskeleton organization
detection of cell density
adipose tissue development
cellular response to anisomycin
motor neuron apoptotic process
amelogenesis
hepatocyte apoptotic process
cellular response to virus
positive regulation of cardiac muscle myoblast proliferation
positive regulation of mitochondrial membrane permeability involved in apoptotic process
intrinsic apoptotic signaling pathway in response to hypoxia
cellular response to leucine starvation
peptidyl-threonine phosphorylation
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