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Invitrogen
Phospho-ATM (Ser1981) Monoclonal Antibody (10H11.E12), eBioscience™
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Product Details
14-9046-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
10H11.E12
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This 10H11.E12 monoclonal antibody recognizes the human, mouse, and rat ataxia-telangiectasia (ATM) protein when phosphorylated on serine 1981. ATM belongs to the family of PI3 kinases and functions by coordinating cell cycle arrest and initiation of DNA repair through the phosphorylation of a multitude of substrates in response to DNA damage and oxidative stress. ATM exists as dimers or multimers in its inactive state but in response to DNA breaks, becomes activated through formation of monomers and autophosphorylation. Activated ATM is recruited to the site of DNA double strand breaks where it halts the cell cycle and initiates DNA repair through the phosphorylation of downstream DNA damage response pathway members. Loss of functional activity of ATM results in ataxia-telangiectasia (AT), a disease characterized by early onset neurodegeneration and predisposition to cancer. AT patients are immunodeficient, radiosensitive, and have an increased risk of certain cancer types such as lymphoma and leukemia.
Applications Reported: This 10H11.E12 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunoprecipitation, western blotting, immunocytochemistry, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. (Fluorochrome conjugated 10H11.E12 is recommended for use in intracellular flow cytometry.).
Applications Tested: This 10H11.E12 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using either high or low pH antigen retrieval and can be used at less than or equal to 10 µg/mL. This 10H11.E12 antibody has been tested by immunocytochemistry of methanol or formaldehyde-fixed and permeabilized cells and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Ataxia-telangiectasia Mutated (ATM) is a protein that belongs to the PI3/PI4 kinase family. Ataxia-telangiectasia is a rare autosomal recessive disorder characterized by progressive neurologic degeneration, immunologic deficiency, and an increased risk of lymphoid cancer. The ATM gene codes for a protein belonging to the phosphoinositide 3-kinase (PI3K) superfamily. ATM phosphorylates proteins instead of lipid and has many downstream targets that act as cell-cycle regulators including: P53, Mdm2, BRCA1, and SMC1. The ATM protein is responsible for repairing double-stranded DNA breaks that occur because of ionizing radiation and other mutagens. The ATM's C-terminal region has extensive homology to the catalytic domains of phosphatidylinositol 3-kinases (PI3 kinases). Studies have shown that ATM becomes autophosphorylated and upregulated by exposure to ionizing radiation. AT cells are hypersensitive to ionizing radiation, impaired in mediating the inhibition of DNA synthesis and display delays in p53 induction. Further, DNA damage caused by ionizing irradiation activates ATM-kinase, leading to a cascade of kinase reactions that regulate cell cycle, apoptosis, and DNA damage repair. Studies have linked ATM to apoptosis along with Nbs1 and Chk2 in the E2F1 pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: A-T mutated; A-T mutated homolog; AT mutated; AT mutated protein; Ataxia t; Ataxia telangiectasia gene mutated in human beings; Ataxia telangiectasia mutated; Ataxia telangiectasia mutated homolog; DKFZp781A0353; EC 2.7.1.37; MGC74674; Serine-protein kinase ATM; TEL1, telomere maintenance 1, homolog
Gene Aliases: AI256621; AT1; ATA; ATC; ATD; ATDC; ATE; ATM; C030026E19Rik; TEL1; TELO1
UniProt ID: (Human) Q13315, (Mouse) Q62388
Entrez Gene ID: (Human) 472, (Mouse) 11920, (Rat) 300711
DNA binding
protein serine/threonine kinase activity
DNA-dependent protein kinase activity
protein binding
ATP binding
1-phosphatidylinositol-3-kinase activity
protein complex binding
protein dimerization activity
protein N-terminus binding
nucleotide binding
protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
phosphotransferase activity, alcohol group as acceptor
histone serine kinase activity
non-receptor serine/threonine protein kinase
double-strand break repair via homologous recombination
DNA double-strand break processing
DNA synthesis involved in DNA repair
strand displacement
response to hypoxia
somitogenesis
pre-B cell allelic exclusion
DNA replication
DNA repair
double-strand break repair via nonhomologous end joining
protein phosphorylation
cellular response to DNA damage stimulus
DNA damage induced protein phosphorylation
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
telomere maintenance via telomerase
cell cycle arrest
mitotic spindle assembly checkpoint
reciprocal meiotic recombination
signal transduction
brain development
heart development
determination of adult lifespan
intrinsic apoptotic signaling pathway in response to DNA damage
response to ionizing radiation
regulation of autophagy
histone phosphorylation
peptidyl-serine phosphorylation
negative regulation of B cell proliferation
positive regulation of telomere maintenance via telomerase
positive regulation of histone phosphorylation
V(D)J recombination
phosphatidylinositol-3-phosphate biosynthetic process
peptidyl-serine autophosphorylation
lipoprotein catabolic process
regulation of apoptotic process
positive regulation of apoptotic process
positive regulation of DNA damage response, signal transduction by p53 class mediator
positive regulation of neuron apoptotic process
meiotic telomere clustering
protein autophosphorylation
oocyte development
neuron apoptotic process
regulation of telomerase activity
histone mRNA catabolic process
cellular response to gamma radiation
cellular response to X-ray
cellular response to nitrosative stress
signal transduction involved in mitotic G2 DNA damage checkpoint
replicative senescence
establishment of RNA localization to telomere
establishment of macromolecular complex localization to telomere
regulation of cellular response to heat
regulation of signal transduction by p53 class mediator
negative regulation of TORC1 signaling
negative regulation of telomere capping
positive regulation of telomere maintenance via telomere lengthening
positive regulation of telomerase catalytic core complex assembly
DNA damage checkpoint
telomere maintenance
cell cycle
female gamete generation
phosphorylation
histone-serine phosphorylation
phosphatidylinositol phosphorylation
regulation of cell cycle
apoptotic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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