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Invitrogen
Phospho-Actin (Tyr53) Polyclonal Antibody
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Phospho-Actin (Tyr53) Antibody (PA5-143643) in ICC/IF
Immunocytochemistry analysis of Phospho-Actin (Tyr53) polyclonal antibody (Product # PA5-143643) and anti-Actin (N-terminal) in C2C12 cells control (left) or treated with pervanadate (1 mM) for 30 min (middle). The cells were fixed in paraformaldehyde and permeabilized in acetone and both antibodies were used in the presence of blocking peptides: Actin (N-terminal) peptide or phospho-Actin (Tyr53) peptide, ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-143643
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Phospho-Actin (Tyr-53) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 53 of human beta actin. This sequence is identical to similar regions in all four alpha actins, as well as in gamma actin, and is well conserved in actins from most eukaryotic species.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
0.25 mg/mL
规格
25 µg
纯化类型
Antigen affinity chromatography
保存液
PBS with 1mg/mL BSA, 50% glycerol
内含物
0.05% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
This antibody detects a 42 kDa* protein corresponding to the molecular mass of Actin on SDS-PAGE immunoblots of pervanadate treated human C2C12 and SYF cSrc transformed cells, but not in control cells. In addition, this antibody either detects actin cleavage products at 36 and 20 kDa, or cross-reacts with unidentified proteins at these molecular weights in C2C12 cells.
靶标信息
Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. In vertebrates there are three groups of actin isoforms: alpha, beta and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exists in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ACT2; actin 2; actin 7; actin alpha 1; actin alpha cardiac; actin alpha cardiac 1; Actin, alpha cardiac muscle 1; Actin, alpha skeletal muscle; actin, alpha, cardiac 1; actin, alpha, vascular smooth muscle; Actin, aortic smooth muscle; Actin, cytoplasmic 1; Actin, cytoplasmic 2; actin, gamma, cytoplasmic 1; Actin, gamma-enteric smooth muscle; alpha-actin cardiac; Alpha-actin-1; Alpha-actin-2; Alpha-actin-3; Alpha-cardiac actin; alpha-sma; alpha-smooth muscle actin; alphac-actin; AtACT7; Atcb; Beta-actin; beta-actin FE-3; Cell growth-inhibiting gene 46 protein; cytoplasmic beta-actin; DEFORMED ROOT HAIRS 1; DER1; ENHANCER OF LRX1 2; ENL2; FIZ2; FRIZZY AND KINKED SHOOTS 2; Gamma-2-actin; Gamma-actin; gamma-enteric smooth muscle actin; LIGHT STRESS-REGULATED 2; LSR2; MYH9.2; MYH9_2; pan actin; RP5-1068B5.2; smooth muscle alpha-actin; Smooth muscle gamma-actin
基因别名: 0610041G09Rik; a-SMA; AA959943; Act-4; Act4; ACTA; Acta-2; ACTA1; ACTA2; ACTA3; ACTB; ACTC; Actc-1; ACTC1; ACTG; ACTG1; ACTG2; ACTGE; Actl; ACTL3; Acts; ACTSA; ACTSG; Actsk-1; ACTVS; Actx; AL023024; alphaSMA; beta-actin; E430023M04Rik; E51; GIG46; SMalphaA; SMGA
UniProt ID: (Mouse) P68134, (Rat) P68136, (Rat) P62738, (Mouse) P62737, (Rat) P60711, (Mouse) P60710, (Mouse) P68033, (Rat) P68035, (Mouse) P63260, (Rat) P63259, (Mouse) P63268, (Rat) P63269
Entrez Gene ID: (Mouse) 11459, (Rat) 29437, (Rat) 81633, (Mouse) 11475, (Rat) 81822, (Mouse) 11461, (Mouse) 11464, (Rat) 29275, (Mouse) 11465, (Rat) 287876, (Mouse) 11468, (Rat) 25365
nucleotide binding
protein binding
ATP binding
protein kinase binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
kinesin binding
Tat protein binding
nucleosomal DNA binding
nitric-oxide synthase binding
ATPase activity
myosin binding
structural constituent of cytoskeleton
ubiquitin protein ligase binding
identical protein binding
molecular_function
actin and actin related protein
actin or actin-binding cytoskeletal protein
cytoskeletal protein
skeletal muscle thin filament assembly
skeletal muscle fiber development
response to mechanical stimulus
response to extracellular stimulus
response to lithium ion
cell growth
skeletal muscle fiber adaptation
response to steroid hormone
muscle contraction
regulation of blood pressure
response to virus
vascular smooth muscle contraction
glomerular mesangial cell development
retina homeostasis
axonogenesis
substantia nigra development
ATP-dependent chromatin remodeling
retina development in camera-type eye
platelet aggregation
cellular response to electrical stimulus
metabolic process
actin filament-based movement
actomyosin structure organization
actin-myosin filament sliding
negative regulation of apoptotic process
cardiac myofibril assembly
cardiac muscle tissue morphogenesis
heart contraction
cardiac muscle contraction
actin-mediated cell contraction
response to drug
response to ethanol
sarcomere organization
response to calcium ion
biological_process
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