Search
GenScript
Phospho-Akt (Ser473) Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
图: 1 / 1
Phospho-Akt (Ser473) Antibody (A00959-40) in WB
Western blot analysis of cell lysates using Akt Antibody (Ser 473 ), pAb, Rabbit (GenScript, A00959, 1 µg/mL). The signal was developed with Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098) and LumiSensor TM HRP Substrate Kit (GenScript, L00221). Predicted Size: 57 kD. Observed Size: 57 kD.
产品信息
A00959-40
应用
建议稀释比
已发表文章
产品规格
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthesized phosphopeptide (KLH conjugated) derived from human Akt around the phosphorylation site of serine 473 (Q-F-S473-Y-S)
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
规格
40 µg
保存条件
-20°C or -80°C if preferred
运输条件
Wet ice
产品详细信息
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Akt, also known as protein kinase B (PKB), a serine/ threonine kinase, is a critical enzyme in several signal transduction pathways involved in cell proliferation, apoptosis, angiogenesis, and diabetes. Akt is activated following its phosphorylation at two regulatory residues. Phosphorylation of threonine on the kinase domain, catalyzed by PDK1, is essential for Akt activation. Akt activity is augmented approximately 10-fold by phosphorylation at the serine on the hydrophobic motif by PDK2. Phosphorylation of Thr308 and Ser473 activates Akt α. Phosphorylation at Thr309 and Ser474 on Akt β1 and β2, and on Thr305 on Akt γ result in their activation. Akt promotes cell survival by inhibiting apoptosis by phosphorylating and inactivating several targets, including Bad, forkhead transcription factors, c-Raf and caspase-9. The activation of Akt is negatively regulated by PTEN, a PIP3 specific phosphatase, and SHIP, a SH2-domain containing inositol 5-phosphatase.GenScript Rabbit Anti-Akt (Ser473) Polyclonal Antibody is developed in rabbit using a synthetic peptide corresponding to residues surrounding S473 of human, rat and mouse Akt α, also known as protein kinase B alpha (PKBα), Akt1, and RACα. In addition, this peptide is identical to the sequence surrounding S474 of human, rat and mouse Akt β (Akt2) and S472 of human and mouse Akt γ (Akt3).
靶标信息
AKT1 (PKB alpha) is a serine/threonine kinase that regulates cell survival. The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating numerous targets in various cell types, including cancer cells. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
