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Invitrogen
Phospho-CD247 (CD3 zeta) (Tyr142) Monoclonal Antibody (3ZBR4S), Alexa Fluor™ 488, eBioscience™
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FIGURE: 1 / 1
Phospho-CD247 (CD3 zeta) (Tyr142) Antibody (53-2478-42) in Flow
Normal human peripheral blood cells were unstimulated (left) or stimulated for 15 minutes with CD3 and CD28 Monoclonal Antibodies, Functional Grade (Product # 16-0037-85) and (Product # 16-0289-85), followed by a 5 minute incubation with F(ab')2-Goat anti-Mouse IgG (H+L) Secondary Antibody, Functional Grade (Product # 16-5098-85) (right). Cells were then stained intracellularly, using the Intracellular Fixa... View More
Product Details
53-2478-42
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG2b, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
3ZBR4S
Immunogen
Human CD247 peptide phosphorylated on Tyr142
Conjugate
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This 3ZBR4S monoclonal antibody recognizes human and mouse CD247 (CD3z) when phosphorylated on Tyrosine 142. CD3 is a multi-protein complex expressed on the surface of thymocytes and T cells, a marker of the T cell lineage, and a critical component of T cell receptor (TCR) signaling. The complex contains a gamma chain, a delta chain, and two epsilon chains, which associate with the alpha/beta or gamma/delta chains of the TCR, and the zeta chain homodimer. The CD3 zeta chain is responsible for initiating the signaling cascade following engagement of the TCR. Each CD3 zeta chain contains three immunoreceptor tyrosine-based activation motifs (ITAM) that are phosphorylated by Src kinases such as Lck. Once phosphorylated, these ITAMs form docking sites for SH2 domain-containing proteins and function to recruit ZAP-70 to the TCR where it can further amplify and propagate signaling. Loss or mutation of CD3 zeta or the ITAM motifs results in impaired T cell development, signaling, and function. CD3 zeta phosphorylation occurs early in the TCR signaling cascade. It occurs immediately downstream of Lck activation, and upstream of ZAP-70, LAT, and SLP-76.
Applications Reported: This 3ZBR4S antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This 3ZBR4S antibody has been pre-diluted and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol A: Two step protocol for intracellular (cytoplasmic) proteins" located at Flow Protocols . This may be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.
Target Information
CD3z complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules, and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. Diseases associated with CD3z include Immunodeficiency 25 and T-B+ Severe Combined Immunodeficiency Due To Cd3delta/Cd3epsilon/Cd3zeta.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD247; CD247 antigen; CD247 antigen, zeta subunit; CD3 antigen, zeta polypeptide; CD3-epsilon; CD3-zeta/eta; CD3e antigen; CD3Z antigen, zeta polypeptide (TiT3 complex); CD3zeta chain; FLJ18683; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor T3 eta chain; T-cell receptor T3 zeta chain; T-cell surface glycoprotein CD3 zeta chain; T3E; TCR zeta chain; TCRE
Gene Aliases: 4930549J05Rik; A430104F18Rik; AW552088; CD247; Cd3; Cd3-eta; CD3-ZETA; CD3H; CD3Q; CD3Z; Cd3zeta; IMD25; T3Z; Tcrk; TCRZ
UniProt ID: (Human) P20963, (Mouse) P24161
Entrez Gene ID: (Human) 919, (Mouse) 12503
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