Invitrogen

Phospho-EEF2 (Tyr443) Polyclonal Antibody

Name: Phospho-EEF2 (Tyr443) Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-105490
Availability: InStock

查看其他25个EEF2抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (5)

Phospho-EEF2 (Tyr443) Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 5

Phospho-EEF2 (Tyr443) Antibody (PA5-105490) in ICC/IF

Immunocytochemistry analysis of Phospho-EEF2 (Tyr443) in H2O2 treated HepG2 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-EEF2 (Tyr443) polyclonal antibody (Product # PA5-105490) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (... View More

Phospho-EEF2 (Tyr443) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-EEF2 (Tyr443) Antibody in Western Blot (WB)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

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Immunogen image

Immunogen Image Phospho-EEF2 (Tyr443) Polyclonal Antibody

产品信息

PA5-105490

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:100-1:500

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human EEF2(Accession P13639), corresponding to amino acid residues around phosphorylated Tyr443.

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

sequential chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2816918

产品详细信息

Antibody detects endogenous levels of EEF2 only when phosphorylated at Tyr443.

靶标信息

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.

仅用于科研。不用于诊断过程。未经明确授权不得转售。