Search
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
Phospho-ERK1/ERK2 (Thr185, Tyr187) Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
图: 1 / 46
Phospho-ERK1/ERK2 (Thr185, Tyr187) Antibody (44-680G) in IHC (P)
Immunohistochemistry analysis of ERK1/2 (pTpY185/187) showing staining in the cytoplasm and nucleus of paraffin-embedded human breast carcinoma tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methan... View More
产品信息
44-680G
应用
建议稀释比
已发表文章
产品规格
种属反应
Bovine,
C. elegans,
Chicken,
Fruit fly,
Human,
Mouse,
Rat,
Xenopus
已发表种属
C. elegans,
Dog,
Human,
Mouse,
Pig,
Rat,
Xenopus
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human ERK1&2 that contains threonine 202/185 and tyrosine 204/187. This region is conserved among many species including rat, mouse, cow, frog, snail, nematode, and fruit fly.
偶联物
形式
Liquid
纯化类型
Antigen affinity chromatography
保存液
Dulbecco's PBS, pH 7.3, with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Purified from rabbit serum by sequential epitope-specific chromatography, this product contains enough material for 10 mini-blots. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the sites of phosphorylation to remove antibody that is reactive with non-phosphorylated ERK1&2. The final product is generated by affinity chromatography using an ERK1&2-derived peptide that is phosphorylated at threonine 202/185 and tyrosine 204/187, respectively, within the activation loop.
This antibody has been used in western blotting and previous lots have been used in immunostaining. Other applications may work but have not been tested. The positive control used in western analysis were PC12 cells +/- Sorbitol; NIH3T3 +/- PDGF; and HEK293 +/- UV.
靶标信息
ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CG12559-PA; CG12559-PC; CG12559-PD; CG12559-PE; CG12559-PG; CG12559-PH; CG12559-PI; Diphospho-ERK; dp-ERK; dpERK; drosophila ERK; enhancer of seven in absentia 7; ERK; Erk MAP kinase; ERK-1; ERK-2; ERK/rolled; erk1 erk2; ERK1b; ERT1; ERT2; extracellular signal-regulated kinase; Extracellular signal-regulated kinase 1; Extracellular signal-regulated kinase 2; extracellular signal-regulated kinase A; extracellular signal-related kinase 1; Extracellular-regulated kinase A; extracellular-signal-regulated kinase 2; Extracellular-signal-related kinase A; Insulin-stimulated MAP2 kinase; M phase MAP kinase; MAP kinase; MAP kinase 1; MAP kinase 2; MAP kinase 3; MAP kinase isoform p42; MAP kinase isoform p44; MAP-kinase; MAPK; MAPK 1; MAPK 2; MAPK 3; MBP kinase; Microtubule-associated protein 2 kinase; mitogen activated protein kinase; mitogen activated protein kinase 1; mitogen-activated 3; mitogen-activated protein kinase; Mitogen-activated protein kinase 1; mitogen-activated protein kinase 1a; Mitogen-activated protein kinase 2; Mitogen-activated protein kinase 3; Mitogen-activated protein kinase ERK-A; MNK1; Myelin basic protein kinase; Myelin xP42 protein kinase; p42; p42 MAP Kinase; p42-MAPK; p44 MAP kinase; p44-ERK1; p44-MAPK; phospho-ERK; pp42/MAP kinase; Protein rolled; protein tyrosine kinase ERK2; RE08694p; rl-PA; rl-PC; rl-PD; rl-PE; rl-PG; rl-PH; rl-PI; roll; sevenmaker
基因别名: 12559; 9030612K14Rik; AA407128; AU018647; BcDNA:RE08694; C78273; CG12559; CG18732; CT34260; CT39192; D-ERK; DERK; DERK-A; dm-dpERK; Dmel\CG12559; Dmel_CG12559; DmERK; DmERK-A; DmERKA; DmMAPK; dp-ERK; DpERK; dpERK1; dpMAPK; Dsor2; E(sina)7; EC2-1; EK2-1; ERK; ERK-1; ERK-2; ERK-A; Erk/Map kinase; ERK1; ERK1/2; ERK2; ERKA; ERT1; ERT2; Esrk1; EY2-2; GroupII; HS44KDAP; HUMKER1A; l(2)41Ac; l(2R)EMS45-39; MAP-k; MAPK; MAPK1; mapk1-a; mapk1-b; mapk1a; MAPK2; MAPK3; MNK1; mpk1; Mtap2k; p-ERK; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; p44; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; pERK; pMAPK; PRKM1; PRKM2; PRKM3; RL; rl/ERK; rl/MAPK; rll; SEM; SR2-1; Su(Raf)2B; XELAEV_18010534mg; xp42
phosphotyrosine binding
protein serine/threonine kinase activity
MAP kinase kinase activity
ATP binding
RNA polymerase II carboxy-terminal domain kinase activity
phosphatase binding
identical protein binding
DNA binding
MAP kinase activity
protein binding
transcription factor binding
kinase activity
mitogen-activated protein kinase kinase kinase binding
scaffold protein binding
nucleotide binding
protein kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
protein kinase binding
non-receptor serine/threonine protein kinase
activation of MAPK activity
protein phosphorylation
apoptotic process
cellular response to DNA damage stimulus
cell cycle
positive regulation of gene expression
positive regulation of peptidyl-threonine phosphorylation
peptidyl-serine phosphorylation
peptidyl-threonine phosphorylation
cytosine metabolic process
regulation of ossification
regulation of cellular pH
thyroid gland development
regulation of protein stability
lipopolysaccharide-mediated signaling pathway
positive regulation of telomere maintenance via telomerase
mammary gland epithelial cell proliferation
cellular response to amino acid starvation
cellular response to reactive oxygen species
response to nicotine
ERBB signaling pathway
outer ear morphogenesis
response to exogenous dsRNA
negative regulation of cell differentiation
thymus development
T cell receptor signaling pathway
B cell receptor signaling pathway
stress-activated MAPK cascade
positive regulation of telomerase activity
Bergmann glial cell differentiation
long-term synaptic potentiation
face development
lung morphogenesis
trachea formation
labyrinthine layer blood vessel development
cardiac neural crest cell development involved in heart development
ERK1 and ERK2 cascade
response to epidermal growth factor
cellular response to cadmium ion
cellular response to tumor necrosis factor
cellular response to granulocyte macrophage colony-stimulating factor stimulus
cellular response to dopamine
positive regulation of telomere capping
MAPK cascade
MAPK import into nucleus
transcription, DNA-templated
chemotaxis
response to stress
signal transduction
chemical synaptic transmission
axon guidance
positive regulation of cell proliferation
fibroblast growth factor receptor signaling pathway
response to toxic substance
regulation of phosphatidylinositol 3-kinase signaling
viral process
sensory perception of pain
platelet activation
positive regulation of cell migration
regulation of stress-activated MAPK cascade
Fc-epsilon receptor signaling pathway
Fc-gamma receptor signaling pathway involved in phagocytosis
response to estrogen
positive regulation of translation
positive regulation of transcription, DNA-templated
regulation of sequence-specific DNA binding transcription factor activity
regulation of cytoskeleton organization
JAK-STAT cascade involved in growth hormone signaling pathway
caveolin-mediated endocytosis
regulation of Golgi inheritance
regulation of cellular response to heat
regulation of early endosome to late endosome transport
positive regulation of protein phosphorylation
transcription initiation from RNA polymerase I promoter
protein complex assembly
DNA damage induced protein phosphorylation
phosphorylation
arachidonic acid metabolic process
BMP signaling pathway
positive regulation of cyclase activity
positive regulation of histone phosphorylation
positive regulation of histone acetylation
peptidyl-tyrosine autophosphorylation
positive regulation of transcription from RNA polymerase II promoter
cartilage development
positive regulation of ERK1 and ERK2 cascade
interleukin-1-mediated signaling pathway
cellular response to mechanical stimulus
positive regulation of xenophagy
negative regulation of apolipoprotein binding
organ morphogenesis
response to lipopolysaccharide
intracellular signal transduction
cellular response to organic substance
mitotic cell cycle
cell surface receptor signaling pathway
epidermal growth factor receptor signaling pathway
terminal region determination
terminal branching, open tracheal system
R7 cell fate commitment
imaginal disc-derived wing vein specification
imaginal disc-derived wing morphogenesis
leg disc proximal/distal pattern formation
metamorphosis
insulin receptor signaling pathway
torso signaling pathway
determination of adult lifespan
cellular response to starvation
regulation of gene expression
negative regulation of macroautophagy
lymph gland plasmatocyte differentiation
lymph gland crystal cell differentiation
tracheal pit formation in open tracheal system
sevenless signaling pathway
positive regulation of cell size
positive regulation of photoreceptor cell differentiation
dorsal appendage formation
vascular endothelial growth factor receptor signaling pathway
behavioral response to ethanol
modulation of synaptic transmission
positive regulation of sequence-specific DNA binding transcription factor activity
defense response to virus
branching involved in open tracheal system development
cellular response to arsenic-containing substance
positive regulation of wound healing
regulation of heart morphogenesis
regulation of response to drug
