Invitrogen

Phospho-Histone H2A.X (Ser139) Monoclonal Antibody (CR55T33), eFluor™ 570, eBioscience™

Name: Phospho-Histone H2A.X (Ser139) Monoclonal Antibody (CR55T33), eFluor\u2122 570, eBioscience\u2122
Brand: Invitrogen
SKU: 41-9865-82

高级验证

3个已发表图片

3 篇参考文献

查看其他76个Histone H2A.X抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

Phospho-Histone H2A.X (Ser139) Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 7

Phospho-Histone H2A.X (Ser139) Antibody (41-9865-82) in ICC/IF

Immunocytochemistry of fixed and permeabilized MCF-7 cells treated with 10 uM camptothecin for 30 minutes (left) or immunohistochemistry of formalin-fixed paraffin embedded human tonsil tissue (right) using 1 µg/mL of Anti-Human/Mouse phospho-H2AX (S139) eFluor® 570. Nuclei are stained with DAPI. Colocalization of signal with DAPI appears pink.

Phospho-Histone H2A.X (Ser139) Antibody in Western Blot (WB)

Phospho-Histone H2A.X (Ser139) Antibody in Immunohistochemistry (IHC)

Product Image Gallery

产品信息

41-9865-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:400

查看2篇已发表文章 2篇已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1 µg/mL

免疫细胞化学 (ICC/IF)

1:50-1:200

产品规格

种属反应

Human, Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

CR55T33

偶联物

eFluor™ 570 eFluor™ 570 eFluor™ 570

激发/发射光谱

556/569 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2573654

产品详细信息

Description: The CR55T33 monoclonal antibody recognizes phosphorylated serine 139 of human and mouse H2AX. H2AX is a member of the H2A histone family that complex with DNA and other histones to form the repeating nucleosome units characteristic of eukaryotic chromatin. Nucleosomes consist of approximately 147 base pairs of DNA wrapped around an octamer of histones composed of two each of the four histone proteins: H2A, H2B, H3 and H4. After induction of DNA damage such as double-strand breaks by irradiation, genotoxic stresses, replication errors or gene recombination, PI3K-like kinases (e.g., ataxia telangiectasia mutated (ATM), ataxia telangiectasia Rad-3-related (ATR), and DNA-dependent protein kinase (DNA-PK) are activated to phosphorylate serine 139 in H2AX. This early phosphorylation event plays a critical role in recruiting proteins involved in DNA repair.

The monoclonal antibody CR55T33 recognizes a single band of approximately 15 kDa on reduced cell lysates from Jurkat cells stimulated with etoposide.

Applications Reported: This CR55T33 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.

Applications Tested: This CR55T33 antibody has been tested by immunocytochemistry of methanol-fixed cells and can be used at less than or equal to 1 µg/mL. This CR55T33 antibody has also been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using high pH antigen retrieval and can be used at less than or equal to 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filter Recommendation: When using this eFluor™ 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor™ 555 or TRITC filter is acceptable.

Excitation: 555 nm; Emission: 570 nm

靶标信息

Histone H2A.X (H2AX) is a member of the histone H2A family which is one of the four core histones making up the nucleosome core particle. In eukaryotes, DNA double strand breaks (DSBs) have been shown to trigger the phosphorylation of serine 139 at the carboxy terminus of histone H2AX resulting in gamma-H2AX. The phosphorylation of H2AX can be detected by Western blotting or immunofluorescence, revealing the frequency of DSBs. The phosphatidylinositol 3-kinases have been implicated in H2AX phosphorylation, but it is unclear if ATM is the primary H2AX kinase or if other members of the family such as DNA-PK and ATR contribute in a similar manner. Structurally, H2A.x contains 143 amino acid residues. Histone H2A.X is considered a basal histone, being synthesized in G1 as well as in S-phase, and its mRNA contains polyA addition motifs and a polyA tail along with the conserved stem-loop and U7 binding sequences involved in the processing and stability of replication type histone mRNAs. There are two forms of Histone H2A.X mRNA, one about 1600 bases long and contains polyA; the other about 575 bases long, lacking polyA. The short form behaves as a replication type histone mRNA, while the longer behaves as a basal type histone mRNA. Histone H2A.X maps to the 11q23.2-q23.3 region of the human chromosome. Histone H2A.x contributes to histone-formation and therefore the structure of DNA. Histone H2A variant H2A.x specifically regulates the interaction of MDC1 (mediator of DNA damage checkpoint protein 1), a DNA repair protein to the sites of DNA damage.

仅用于科研。不用于诊断过程。未经明确授权不得转售。