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Invitrogen
Phospho-LCK (Tyr505) Recombinant Mouse Monoclonal Antibody (SRRCHA), Alexa Fluor™ Plus 647
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Phospho-LCK (Tyr505) Antibody (740076MP647) in ICC/IF
Immunofluorescence analysis of Phospho-LCK (Tyr505) was performed using Phospho-LCK (Tyr505) Recombinant Mouse Monoclonal Antibody (SRRCHA) Alexa Fluor™ Plus 647 (Product # 740076MP647) on Jurkat cells treated with Sodium Pervanadate (1 mM for 5 min). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes and blocked with 2% BSA for 1 hr at RT. The ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
740076MP647
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1, kappa
Expression System
ExpiCHO
分类
Recombinant Monoclonal
类型
Antibody
克隆号
SRRCHA
偶联物
Alexa Fluor™ Plus 647
Alexa Fluor™ Plus 647
Alexa Fluor™ Plus 647
激发/发射光谱
658/675 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
规格
50 µg
纯化类型
Protein A/G
保存液
proprietary buffer, pH 6.3-7.3
内含物
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Wet ice
产品详细信息
Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 658 nm; Emission: 675 nm; Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
LCK is a member of the Src-family tyrosine kinase, which are essential for signaling through the T-cell receptor (TCR) complex. Upon TCR triggering, LCK phosphorylates the ITAM motives in its zeta subunits establishing binding sites for the SH2 domains of the tyrosine kinase ZAP70, which is also phosphorylated by LCK and activated to generate subsequent signaling platforms by phosphorylation of adaptor LAT. The majority of LCK is localized to the plasma membrane, however, there is also a significant fraction associated with the Golgi apparatus which may contribute to Raf activation under conditions of weak stimulation through the TCR. LCK contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. LCK is also involved in the regulation of apoptosis induced by various stimuli, but not by the death receptors. Diseases associated with LCK include Immunodeficiency 22 and CD45 deficiency. Alternatively splice variants of the LCK gene encoding different isoforms of the protein have been found.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: EC 2.7.10.2; kinase Lck; leukocyte C-terminal Src kinase; lymphocyte cell-specific protein-tyrosine kinase; OTTHUMP00000008640; OTTHUMP00000008740; OTTHUMP00000008741; p56(LSTRA) protein-tyrosine kinase; proto-oncogene tyrosine-protein kinase LCK; RP4-675E8.4; t cell-specific protein-tyrosine kinase; T-lymphocyte specific protein tyrosine kinase p56lck; tyr; unnamed protein product
基因别名: IMD22; LSK; p56lck; pp58lck; YT16
UniProt ID: (Human) P06239
Entrez Gene ID: (Human) 3932
phosphotyrosine binding
protein kinase activity
protein tyrosine kinase activity
non-membrane spanning protein tyrosine kinase activity
protein serine/threonine phosphatase activity
receptor binding
protein binding
ATP binding
phospholipase activator activity
protein kinase binding
protein phosphatase binding
SH2 domain binding
T cell receptor binding
CD4 receptor binding
CD8 receptor binding
identical protein binding
phospholipase binding
phosphatidylinositol 3-kinase binding
ATPase binding
protein antigen binding
immune response-activating cell surface receptor signaling pathway
transmembrane receptor protein tyrosine kinase signaling pathway
response to xenobiotic stimulus
hemopoiesis
platelet activation
T cell differentiation
T cell costimulation
positive regulation of heterotypic cell-cell adhesion
intracellular signal transduction
Fc-gamma receptor signaling pathway
T cell activation
T cell receptor signaling pathway
B cell receptor signaling pathway
positive regulation of T cell receptor signaling pathway
positive regulation of T cell activation
leukocyte migration
NK T cell activation
release of sequestered calcium ion into cytosol
positive regulation of multicellular organismal process
regulation of lymphocyte activation
CD27 signaling pathway
positive regulation of leukocyte cell-cell adhesion
positive regulation of intrinsic apoptotic signaling pathway
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