Invitrogen

Phospho-LIMK1/LIMK2 (Ser323, Ser314) Polyclonal Antibody

Name: Phospho-LIMK1\/LIMK2 (Ser323, Ser314) Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-143714

查看其他7个LIMK1/LIMK2抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)

Phospho-LIMK1/LIMK2 (Ser323, Ser314) Antibody in Western Blot (WB)

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Phospho-LIMK1/LIMK2 (Ser323, Ser314) Antibody (PA5-143714) in WB

Western blot of Phospho-LIMK1/LIMK2 (Ser323, Ser314) in human A431 cells untreated (lanes 1 & 3) or treated (lanes 2 & 4)with calyculin A (100 nM for 30 minutes). The blots were probed with anti-LIMK1 (C-terminus) (lanes 1 & 2) or Phospho-LIMK1/LIMK2 (Ser323, Ser314) polyclonal antibody (Product # PA5-143714) (lanes 3 & 4).

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产品信息

PA5-143714

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

LIMK1 (Ser-323) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding serine 323 in human LIMK1. This sequence is conserved in rat and mouse LIMK1, and has high homology to Ser-314 in human LIMK2.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA, 50% glycerol

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2942942

产品详细信息

This antibody was cross-adsorbed to unphosphorylated LIMK1 (Ser-323) peptide then affinity purified using LIMK1 (Ser-323) peptide (without carrier). The antibody detects a protein with the same mobility as anti-LIMK1 (C-terminus, LP1831) in human A431 cells treated with calyculin A. This band is weak in control cells and is not detected after lambda phosphatase treatment.

靶标信息

Proteins containing LIM motifs are typically involved in cell fate determination and growth control. A family of proteins designated LIM kinases, including LIMK-1 and LIMK-2, has been identified. LIMK-1 has been shown to regulate the stabilization of F-Actin structures and cofilin activity, indicating that LIMK-1 plays a role in a signaling pathway involved in the regulation of cell motility and morphogenesis. LIMK-1 inhibits neuronal differentiation of PC12 cells, and is thought to act by interfering with events downstream of MAPK activation. Expression patterns of LIMK-1 and LIMK-2 suggest that these proteins may have different functions during development. A truncated form of LIMK-2 has been identified in adult testis that is thought to arise from an alternative initiation exon.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: KIZ-1; LIM domain kinase 1; LIM domain kinase 2; lim kinase 1; LIM kinase 2; LIM motif-containing protein kinase; LIM motif-containing protein kinase 1; LIM motif-containing protein kinase 2; LIM-domain containing protein kinase 1; LIM-domain containing, protein kinase 1; LIMK 2; LIMK-1; LIMK-2

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基因别名: KIZ-1; LIMK; LIMK-1; Limk-2; LIMK1; LIMK2; Limk2b; Link2